Copper, nickel, and zinc cations biosorption properties of Gram-positive and Gram-negative MerP mercury-resistance proteins
學年 106
學期 1
出版（發表）日期 2017-11-01
作品名稱 Copper, nickel, and zinc cations biosorption properties of Gram-positive and Gram-negative MerP mercury-resistance proteins
作品名稱（其他語言）
著者 Yi-Huang Hsueh, Kuen-Song Lin, Yu-Ting Wang, Chao-Lung Chiang
單位
出版者
著錄名稱、卷期、頁數 Journal of the Taiwan Institute of Chemical Engineers 80, p.168-175
摘要 The mercury resistance (mer) operon is present in a wide range of bacteria but relevant research has been conducted mainly on Gram-negative bacteria. We compared the heavy metal biosorption properties of Gram-positive and Gram-negative MerP, a key metal-binding transporter of the mer operon that scavenges heavy metal ions in the periplasm for subsequent neutralization in the cytoplasm by other Mer proteins. The MerP proteins of the Gram-positive Bacillus cereus RC607 and Gram-negative Pseudomonas aeruginosa K-62 strains in Escherichia coli strain BL21 were expressed to derive the recombinant strains BL21+MerPBc and BL21+MerPPa, respectively. The BL21+MerPBc having a higher affinity for Ni2+ and Cu2+ ions than that of Zn2+ ions was found, whereas BL21+MerPPa preferentially adsorbed Zn2+ ions over Ni2+ and Cu2+ ions. Furthermore, X-ray photoelectron spectroscopy revealed that regardless of the initial ion concentration, Zn, Ni, and Cu were mainly present as oxides in BL21+MerPBc but were mainly present as sulfides at low ion concentrations in BL21+MerPPa, with oxides primarily detected only when the initial ion concentrations were high. These findings were confirmed through X-ray absorption near edge structure and extended X-ray absorption fine structure analyses. These results have revealed that the heavy metal biosorption properties differ between Gram-positive and Gram-negative MerP proteins.
關鍵字
語言 en
ISSN
期刊性質 國外
收錄於 SCI SSCI Scopus
產學合作
通訊作者 Yi-Huang Hsueh, Kuen-Song Lin
審稿制度 是
國別 NLD
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/127464 )

SDGS 永續城市與社區,負責任的消費與生產,陸域生命,良好健康和福祉,潔淨水與衛生