Recovery of Candidatus Brocadia fulgida Persisted After a 3-Month Starvation Period: Nitrogen Removal Performance and Microbial Marker Identification
學年 115
學期 1
出版（發表）日期 2026-10-20
作品名稱 Recovery of Candidatus Brocadia fulgida Persisted After a 3-Month Starvation Period: Nitrogen Removal Performance and Microbial Marker Identification
作品名稱（其他語言）
著者 Wannakarn Sonkhum; Jarawee Kaewyai; Sumeth Wongkiew; Chi-Wang Li; Pongsak (Lek) Noophan
單位
出版者
著錄名稱、卷期、頁數 Water Environment Research 98(10), e70632
摘要 The recovery of partial nitritation and anammox (PN/A) process after starvation remains challenging. Substrate limitation disrupts microbial activity and delays the restoration of nitrogen removal performance. Therefore, PN/A nitrogen removal performance was evaluated in a 20-L laboratory-scale continuous stirred-tank reactor (CSTR) containing sponge carriers across four operational phases. The four phases included 3-month starvation, gradual substrate reintroduction under suspended-growth conditions, transitional stabilization with sponge media, and attached-growth recovery under higher nitrogen loading without external acetate supplementation. The laboratory reactor was operated at a nitrogen loading rate of 0.4–0.7 kg N/(m3·d), dissolved oxygen below 0.1 mg/L, and without organic carbon addition. The nitrogen removal efficiency, microbial community composition, and microbial markers of the laboratory-scale reactor were compared with those of two well-operated full-scale systems in Korea and the United States. Total nitrogen removal efficiency exceeded 90% across all three systems when the ammonia-to-nitrite ratio (NH₄+:NO2−) was maintained between 1:1 and 1:1.5 at low BOD/TN ratio of < 0.05. Next-generation sequencing (Illumina MiSeq, 16S rRNA amplicon profiling at a sequencing depth of 41,120–57,097 reads/sample) was employed to determine the relative abundance of nitrogen-transforming microbial communities, specifically the anammox group, across the three systems. The results showed that the anammox bacteria in all three systems belonged to Candidatus Brocadia. Following gene-specific PCR amplification of the anammox community, denaturing gradient gel electrophoresis (DGGE) identified Candidatus Brocadia fulgida. This species exhibits high environmental adaptability and is commonly detected in full-scale systems operated for > 5 years. Molecular analysis indicated that Candidatus Brocadia fulgida persisted in the system throughout the 3-month starvation period, facilitating the re-establishment of the attached-growth PN/A process. The successful recovery of the laboratory-scale reactor yielded nitrogen removal performance comparable to that of the full-scale PN/A systems, demonstrating that the Brocadia group serves as a keystone taxon during process recovery.
關鍵字
語言 en_US
ISSN 1554-7531
期刊性質 國外
收錄於 SCI
產學合作
通訊作者
審稿制度 否
國別 USA
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/129998 )