Zebrafish PRL-3 regulates yolk syncytial layer integrity and actomyosin contractility during epiboly
學年 114
學期 2
出版（發表）日期 2026-03-02
作品名稱 Zebrafish PRL-3 regulates yolk syncytial layer integrity and actomyosin contractility during epiboly
作品名稱（其他語言）
著者 Ting-Fang Wang; Kai-Wen Cheng; Yau-Hung Chen; Ming-Der Lin
單位
出版者
著錄名稱、卷期、頁數 International Journal of Molecular Sciences 27(5), p. 2339
摘要 Abstract Zebrafish epiboly is a critical morphogenetic event driven by the precise coordination of microtubule-mediated pulling forces and actomyosin-dependent constriction. While the phosphatase PRL-3 is known to regulate cytoskeletal remodeling in cancer metastasis, its physiological function during early vertebrate embryogenesis remains undefined. Here, we identify zfPRL-3 as an indispensable regulator of zebrafish epiboly. Morpholino-mediated depletion of zfPRL-3 resulted in severe developmental arrest, blastoderm destabilization, and mechanical rupture of the yolk cell. Time-lapse imaging revealed that zfPRL-3 morphants suffer from catastrophic structural failures, characterized by either blastoderm dispersion or excessive inward constriction. At the cellular level, we demonstrate that zfPRL-3 depletion disrupts the organization of the Yolk Syncytial Layer (YSL), evidenced by the irregular scattering of YSL nuclei—a hallmark of microtubule network collapse. Furthermore, zfPRL-3 morphants exhibit premature assembly of the contractile actomyosin ring at 60% epiboly, indicating a failure in the inhibitory mechanisms that normally restrain marginal constriction. We propose that zfPRL-3 functions as a molecular brake that couples YSL integrity with the timing of contractility. By maintaining microtubule stability and preventing premature actomyosin ring formation, zfPRL-3 ensures that the opposing physical forces driving epiboly are precisely balanced. Collectively, our findings define zfPRL-3 as a critical spatiotemporal regulator that orchestrates the successful progression of epiboly.
關鍵字 phosphatase of regenerating liver-3; epiboly; premature constriction
語言 en_US
ISSN
期刊性質 國外
收錄於 SCI SSCI
產學合作
通訊作者
審稿制度 否
國別 CHE
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/129801 )