Dual-site recognition of sialic acid using an amphiphilic bis‑boronic acid probe in a liquid crystal sensing platform
學年 114
學期 1
出版（發表）日期 2026-01-20
作品名稱 Dual-site recognition of sialic acid using an amphiphilic bis‑boronic acid probe in a liquid crystal sensing platform
作品名稱（其他語言）
著者 Rajib Nandi; Yi-Yun Yeh; Po-Shen Pan; Chih-Hsin Chen
單位
出版者
著錄名稱、卷期、頁數 Microchemical Journal 221 ,p. 117067
摘要 Sialic acid, a terminal monosaccharide present on glycoproteins and glycolipids, plays critical roles in cell adhesion, immune recognition, and metastasis, and its abnormal levels are closely associated with disease progression. Herein, we report a liquid crystal (LC)-based optical sensing platform for label-free detection of sialic acid using a newly designed amphiphilic bis‑boronic acid probe (DIBA) that enables dual-site recognition and enhanced binding affinity. When doped into the nematic liquid crystal 4-cyano-4′-pentylbiphenyl (5CB), DIBA induces homeotropic alignment at the LC/aqueous interface, resulting in a dark optical state. Upon exposure to sialic acid, cooperative binding interactions—primarily boronate ester formation with the α-hydroxycarboxyl group, assisted by hydrogen bonding with the glycerol side chain—disrupt the LC alignment and trigger a distinct dark-to-bright optical transition. The binding mechanism was systematically validated by zeta potential measurements, Fourier transform infrared (FTIR) spectroscopy, binding constant determination, and density functional theory (DFT) calculations. The sensor exhibits excellent selectivity against common monosaccharides, a lowest detectable concentration of 25 μM, and a linear response range of 25–500 μM in buffer. Reliable detection down to 50 μM was further demonstrated in spiked human serum albumin samples, indicating good tolerance toward complex biological matrices. This work represents the first LC-based sensing platform for sialic acid detection employing a bis‑boronic acid amphiphilic probe, providing a simple, non-enzymatic, and visually readable approach for bioanalytical sensing applications.
關鍵字
語言 en
ISSN
期刊性質 國外
收錄於 SCI Scopus
產學合作
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審稿制度 是
國別 BES
公開徵稿
出版型式 ,電子版,紙本
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機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/129198 )