Effects of antigen dosage and chitosan micro/nanoparticle size on immune responses in mice immunized with H5N1 influenza vaccine
學年 114
學期 2
出版（發表）日期 2026-03-05
作品名稱 Effects of antigen dosage and chitosan micro/nanoparticle size on immune responses in mice immunized with H5N1 influenza vaccine
作品名稱（其他語言）
著者 Nguyen AD, Nguyen YN, Pham H, Ha TDL, Nguyen HL, Le L, Nguyen VB, Nguyen DS, Dinh HH, Wang SL*, Cao V
單位
出版者
著錄名稱、卷期、頁數 Polymers, 18(5): 642
摘要 Highly pathogenic avian influenza A/H5N1 remains a persistent threat to public health and poultry production. H5N1 antigens are typically poorly immunogenic and require effective adjuvants for antigen dose-sparing. Here, we evaluated chitosan microparticles (CSMs) and nanoparticles (CSNs) as polymeric nano-adjuvants for an H5N1 influenza vaccine, focusing on the roles of antigen dose and particle size. A purified hemagglutinin antigen was adsorbed onto chitosan particles at doses ranging from 0.15 to 5.0 µg. Both CSNs and CSMs showed consistently high loading efficiency (97–99%). BALB/c mice were immunized intramuscularly in a prime–boost schedule. Chitosan nanoparticles significantly enhanced IgG and hemagglutination inhibition (HI) titers at low antigen doses compared with aluminum hydroxide and antigen-only controls (p < 0.05). Immune responses reached saturation at a 1.5 µg dose of antigen for chitosan nanoparticles and 3.0 µg for chitosan microparticles. IgG subtype analysis suggested a balanced IgG1/IgG2a profile. Collectively, these findings support chitosan-based polymeric nanoparticles as promising adjuvants enabling dose-sparing H5N1 vaccination.
關鍵字 chitosan; nanoparticles; microparticles; polymeric adjuvant; H5N1 influenza vaccine; hemagglutination inhibition; antigen dose-sparing
語言 en
ISSN
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Wang, San-Lang
審稿制度 是
國別 CHE
公開徵稿
出版型式 ,電子版