Utilization of agro-processing by-products for Bacillus velezensis EB.KN15 fermentation and elucidation of its potential fungicide effect
學年 114
學期 1
出版（發表）日期 2026-01-15
作品名稱 Utilization of agro-processing by-products for Bacillus velezensis EB.KN15 fermentation and elucidation of its potential fungicide effect
作品名稱（其他語言）
著者 Ngo VA, Wang SL*, Nguyen VB*, Tran THT, Doan MD, Phan TQ, Phan TKT, Nguyen TH, Nguyen AD*
單位
出版者
著錄名稱、卷期、頁數 Vietnam Journal of Science and Technology
摘要 Agro-processing by-products have been widely used for fermentation to produce bacteria and their bioactive compounds. The aim of this work was to reuse agro by-products as a substrate to produce a bacterial strain Bacillus velezensis EB.KN15 and reporting its potential fungicide effect against various pathogenic fungi. Groundnut oil cake (GOC) was newly investigated as a potential substrate for B. velezensis EB.KN15 fermentation. This substrate contained significant amount of protein (35.7%), lipid (14.3%), total ash (12.34%), and diverse mineral elements. The suitable medium was investigated containing 2% C/N source (mixture of GOC/LB = 2/1), initial pH medium at 6.5, salt compositions of 0.05% CaSO4.2H2O and 0.1% K2HPO4.3H2O, fermented at 34oC, shaking speed at 200 rpm for 36 h. The utilization of a 14 L-bioreactor system for fermentation resulted in harvesting higher bacterial productivity (7.94×1012 CFU/mL) in a much shorter cultivation time (10 h) compared to fermentation in the minor flasks (3.018×109 CFU/mL after 36 h). In bioassays, B. velezensis EB.KN15 showed potential antifungal activity against various pathogenic fungal strains. Of these, Fusarium oxysporum was the most significantly inhibited at a value of 68.18%. The bioactive compounds produced by EB.KN15 was identified using GCMS, and their mechanism interaction was further investigated using a molecular docking study. B. velezensis EB.KN15 was also found producing 2 major plant growth-promoting compounds, including gibberellic acid (GA3) and zeatin with contents of 1589 µg/mL and 30.84 µg/mL, respectively and minor amount of indole-3-acetic acid (IAA) and kinetin. The results suggested that GOC could be used as a promising substrate to produce B. velezensis EB.KN15 for applying as a biocontrol agent in biofungicides and biofertilizers.
關鍵字 Agro by-products, Bacillus velezensis, Fusarium oxysporum, groundnut oil cake, pathogen fungi
語言 en
ISSN
期刊性質 國外
收錄於 Scopus
產學合作
通訊作者 San-Lang, Wang
審稿制度 1
國別 VNM
公開徵稿
出版型式 ,電子版