Enzymatic production of chitooligosaccharide using a GH family 46 chitosanase from Paenibacillus elgii and its antioxidant activity
學年 113
學期 1
出版（發表）日期 2024-10-29
作品名稱 Enzymatic production of chitooligosaccharide using a GH family 46 chitosanase from Paenibacillus elgii and its antioxidant activity
作品名稱（其他語言）
著者 Wang San-lang
單位
出版者
著錄名稱、卷期、頁數 Catalysts, 14(11): 761
摘要 first_pagesettingsOrder Article Reprints Open AccessArticle Enzymatic Production of Chitooligosaccharide Using a GH Family 46 Chitosanase from Paenibacillus elgii and Its Antioxidant Activity by Chien Thang Doan 1,*ORCID,Thi Ngoc Tran 1ORCID,Anh Dzung Nguyen 2ORCID andSan-Lang Wang 3,4,*ORCID 1 Faculty of Natural Science and Technology, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam 2 Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam 3 Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan 4 Life Science Development Center, Tamkang University, New Taipei City 25137, Taiwan * Authors to whom correspondence should be addressed. Catalysts 2024, 14(11), 761; https://doi.org/10.3390/catal14110761 Submission received: 29 August 2024 / Revised: 15 October 2024 / Accepted: 24 October 2024 / Published: 29 October 2024 (This article belongs to the Special Issue Enzyme and Biocatalysis Application) Downloadkeyboard_arrow_down Browse Figures Versions Notes Abstract Chitooligosaccharide (COS), a natural antioxidant, is a hydrolysis product of chitosan created using enzymatic or chemical methods. COS has received considerable attention recently, making its efficient bioproduction of great value. This study investigated the optimal conditions for the enzymatic method using a GH family 46 chitosanase from Paenibacillus elgii TKU051 to prepare COS based on the response surface methodology (RSM). The results showed optimal values for chitosan hydrolysis, such as a pH of 5.5, an incubation temperature of 58.3 °C, an [E]/[S] ratio of 118.494 (U/g), and an incubation time of 6.821 h. Under the optimal conditions, the highest reducing sugar level (per substrate, w/w) of the chitosan hydrolysis process that could be reached was 690.587 mg/g. The composition of the obtained COS was analyzed using the thin-layer chromatography (TLC) method, yielding (GlcN)2 and (GlcN)3 as the products. The ascorbic acid equivalent antioxidant capacity (AEAC) of the obtained COS was found to be 1246 mg/100 g (via a DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging assay) and 3673 mg/100 g (via an ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical-scavenging assay). This green and efficient bioproduction method may possess excellent potential for application in bioactive COS preparation.
關鍵字 antioxidant; chitosan; chitosanase; oligosaccharide; Paenibacillus elgii
語言 en
ISSN 2073-4344
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Wang, San-Lang
審稿制度 是
國別 CHE
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/126435 )