A novel liquid crystal aptasensor via DNA aptamer conformational change for on-site detection of cocaine in sewage
學年 112
學期 1
出版（發表）日期 2023-11-30
作品名稱 A novel liquid crystal aptasensor via DNA aptamer conformational change for on-site detection of cocaine in sewage
作品名稱（其他語言）
著者 Chen, Chih-hsin
單位
出版者
著錄名稱、卷期、頁數 Sensors and Actuators B: Chemical 401,　135076
摘要 A novel one-step liquid crystal (LC)-based aptasensor has been developed for on-site detection of trace substances in sewage analysis. The platform utilizes different mechanisms to simultaneously trigger the upper and bottom layers of LC orientation. By employing trace amounts of CTAB in Tris-HCl buffer, the upper layer of LC is aligned, while DNA aptamers modified on a single glass induce the orientation of the bottom layer. When targeting cocaine, the asymmetric single-stranded DNA aptamers transition to a symmetric Y-shape, causing the reorientation of LCs from planar to homeotropic. This results in an optical change from bright to dark in the LC image. The sensing procedure involves adding pre-buffered samples at pH 7.4 to the system in one step. The platform requires only 10 ng of DNA aptamers, which can be kept dry. It enables on-site detection of cocaine in sewage, exhibiting a linear calibration curve from 1 pM to 15 µM in water (R2 = 0.997) with a limit of detection of 0.5 pM. The aptasensor demonstrates quantitative recoveries ranging from 89.6% to 110.9% in sewage samples. This sensitive, robust, and portable LC-based aptasensor shows promise as a tool for on-site detection of trace substances.
關鍵字 Liquid crystal-based aptasensor;One-step device;On-site detection;Cocaine;Sewage
語言 zh_TW
ISSN 1873-3077
期刊性質 國內
收錄於 SCI
產學合作
通訊作者
審稿制度 否
國別 TWN
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/125892 )