Knockdown of klotho leads to cell movement impairment during zebrafish gastrulation
學年 112
學期 1
出版（發表）日期 2023-08-29
作品名稱 Knockdown of klotho leads to cell movement impairment during zebrafish gastrulation
作品名稱（其他語言）
著者 Heng-Chih Pan; Heng-Chih Pan; Kang-Chieh Lo; Yun-Hsin Wang; Han-Ting Huang; Shu-Chun Cheng; Chiao-Yin Sun; Yau-Hung Chen
單位
出版者
著錄名稱、卷期、頁數 Fishes 8(9), 440
摘要 (1) Background: Klotho is a multifunctional protein, but its biophysiological roles during fish early development are unclear. (2) Methods: We carried out anti-sense Klotho morpholino injection and whole-mount in situ hybridization experiments in this study. (3) Results: Our results showed that in the Klotho MO1-injected group, embryos displayed longitudinal shapes and swelling yolks compared to those of the un-injected groups. Their length/width ratio by 9 hpf for the uninfected group is 1 ± 0.1; but for 0.1 mM-, 0.2 mM-, and 0.5 mM-MO-injected are 1.1 ± 0.1, 1.2 ± 0.1, and 1.3 ± 0.1, respectively, suggesting that the klotho knockdown-induced length/width ratio changes are in a dose-dependent manner. Whole-mount in situ hybridization showed that several cell migration-related gene expressions, including goosecoid, floating head, bone morphogenetic protein 4, and even-skipped-liked 1 were affected in the Klotho MO-injected embryos. (4) Conclusions: We suggest that knockdown of klotho leads to cell movement impairment during zebrafish gastrulation.
關鍵字 klotho; cell movement; zebrafish; embryogenesis
語言 en_US
ISSN 2410-3888
期刊性質 國外
收錄於 SCI SSCI
產學合作
通訊作者
審稿制度 是
國別 CHE
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/125611 )