Chitosanase Production from the Liquid Fermentation of Squid Pens Waste by Paenibacillus elgii
學年 112
學期 1
出版(發表)日期 2023-09-11
作品名稱 Chitosanase Production from the Liquid Fermentation of Squid Pens Waste by Paenibacillus elgii
著者 Wang, San-lang
著錄名稱、卷期、頁數 Polymers 15(18), 3724
摘要 Chitosanases play a significant part in the hydrolysis of chitosan to form chitooligosaccharides (COS) that possess diverse biological activities. This study aimed to enhance the productivity of Paenibacillus elgii TKU051 chitosanase by fermentation from chitinous fishery wastes. The ideal parameters for achieving maximum chitosanase activity were determined: a squid pens powder amount of 5.278% (w/v), an initial pH value of 8.93, an incubation temperature of 38 °C, and an incubation duration of 5.73 days. The resulting chitosanase activity of the culture medium was 2.023 U/mL. A chitosanase with a molecular weight of 25 kDa was isolated from the culture medium of P. elgii TKU051 and was biochemically characterized. Liquid chromatography with tandem mass spectrometry analysis revealed that P. elgii TKU051 chitosanase exhibited a maximum amino acid identity of 43% with a chitosanase of Bacillus circulans belonging to the glycoside hydrolase (GH) family 46. P. elgii TKU051 chitosanase demonstrated optimal activity at pH 5.5 while displaying remarkable stability within the pH range of 5.0 to 9.0. The enzyme displayed maximum efficiency at 60 °C and demonstrated considerable stability at temperatures ≤40 °C. The presence of Mn2+ positively affected the activity of the enzyme, while the presence of Cu2+ had a negative effect. Thin-layer chromatography analysis demonstrated that P. elgii TKU051 chitosanase exhibited an endo-type cleavage pattern and hydrolyzed chitosan with 98% degree of deacetylation to yield (GlcN)2 and (GlcN)3. The enzymatic properties of P. elgii TKU051 chitosanase render it a promising candidate for application in the production of COS.
關鍵字 chitinous fishery wastes;chitosan;chitosanase;chitooligosaccharide;Paenibacillus elgii;squid pens
語言 en
ISSN 2073-4360
期刊性質 國外
收錄於 SCI
通訊作者 San-Lang Wang
國別 CHE
出版型式 ,電子版

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