Detection of okadaic acid using a liquid crystal-based aptasensor by exploiting the signal enhancement effect of gold nanoparticles
學年 111
學期 1
出版（發表）日期 2022-09-01
作品名稱 Detection of okadaic acid using a liquid crystal-based aptasensor by exploiting the signal enhancement effect of gold nanoparticles
作品名稱（其他語言）
著者 Zeinab Rouhbakhsh; Tsung Yang Ho; Asma Verdian; Chih-Hsin Chen
單位
出版者
著錄名稱、卷期、頁數 Biosens. Bioelecton. X 11, 100148
摘要 In this work, a liquid crystal (LC)-based aptasensor for okadaic acid (OA) detection was developed with a novel aptamer mechanism for enhancing the optical signals of LCs. In this molecular strategy, a short linker aptamer was utilized to immobilize on the sensing platform to form a double-stranded structure with OA specific aptamer. The duplex forming disrupted the orientation of LCs and led to bright LC images under polarized light. The brightness of the LC images was enhanced by integrating a signal amplifier aptamer linked with gold nanoparticles (SA-AuNPs) to form an enlarged double-stranded aptamer structure. In the presence of OA, the specific binding between aptamer and OA led to dissociation of double-stranded aptamer structure and released the specific aptamer and SA-AuNPs from the substrate. As a result, the orientation of LC cannot be disrupted and dark LC images were observed. By measuring the brightness of the LC images, the limit of detection of the developed LC-based aptasensor for OA was 0.42 pM with good linearity and selectivity. In addition, we also demonstrated the applicability of this LC-based aptasensor to detect OA in clam samples with good recoveries.
關鍵字 Liquid crystal;Aptamer;Aptasensor;Okadaic acid;Signal amplification
語言 zh_TW
ISSN 2590-1370
期刊性質 國內
收錄於 SCI
產學合作
通訊作者
審稿制度 是
國別 TWN
公開徵稿
出版型式 ,電子版,紙本
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/123402 )