Novel nematocidal compounds from shrimp shell wastes valorized by Bacillus velezensis RB.EK7 against black pepper nematodes
學年 111
學期 1
出版（發表）日期 2022-09-25
作品名稱 Novel nematocidal compounds from shrimp shell wastes valorized by Bacillus velezensis RB.EK7 against black pepper nematodes
作品名稱（其他語言）
著者 Trinh THT; Wang SL; Nguyen VB; Phan TQ; Doan MD; Tran TPH; Nguyen TH; Le TAH; Ton TQ; Nguyen AD
單位
出版者
著錄名稱、卷期、頁數 Agronomy 12(10), 2300
摘要 Among various organic wastes, shrimp shell powder (SSP) was the most suitable carbon/nitrogen source for producing antinematode compounds (ANCs) via Bacillus veleznesis RB.EK7 fermentation. The fermentation process for the enhancement of antinematode activity was investigated. B. veleznesis RB.EK7 produced the highest antinematode activity in the medium containing 0.8% SSP with an initial pH of 6.5–7.0, and fermentation was performed at 35–37 °C with a saking speed of 150 rpm for 72 h. Targeting ANCs were purified from the fermented culture broth and identified as thymine (1) and hexahydropyrrolo [1,2-a]pyrazine-1,4-dione (2) based on nuclear magnetic resonance (NMR) and mass spectra analysis and were compared to those of the reported compounds. Notably, for the first time, these compounds were found as novel ANCs. Thymine (1) demonstrated a potential nematicidal effect with near 100% mortality of second-stage juvenile (J2) nematodes and anti-egg hatching effects of 70.1%, while hexahydropyrrolo [1,2-a]pyrazine-1,4-dione showed moderate antinematode activities with 64.2% mortality of J2 nematodes and anti-egg hatching effects of 57.9%. The docking study coupled with experimental enzyme inhibition results indicated that the potent nematicidal effect of these compounds may be possibly due to the inhibition of the targeting enzyme acetylcholinesterase. The data of this study suggest that SSP can be potentially reused for the eco-friendly production of ANCs for the management of black pepper nematodes.
關鍵字 black pepper;Bacillus veleznesis;nematodes;antinematode compounds;organic wastes;microbial fermentation;thymine
語言 en
ISSN 2073-4395
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 San-Lang, Wang
審稿制度 是
國別 CHE
公開徵稿
出版型式 ,電子版
SDGS 消除貧窮,消除飢餓,良好健康和福祉,潔淨水與衛生,可負擔的潔淨能源