Conversion of fishery waste to proteases by Streptomyces speibonae and their application in antioxidant preparation
學年 110
學期 2
出版（發表）日期 2022-06-14
作品名稱 Conversion of fishery waste to proteases by Streptomyces speibonae and their application in antioxidant preparation
作品名稱（其他語言）
著者 Tran TN; Doan CT; Nguyen VB; Nguyen AD; Wang SL
單位
出版者
著錄名稱、卷期、頁數 Fishes 7(3), 140
摘要 Proteinaceous wastes from the fishery process are an abundant renewable resource for the recovery of a variety of high-value products. This work attempted to utilize several proteinaceous wastes to produce proteases using the Streptomyces speibonae TKU048 strain. Among different possible carbon and nitrogen sources, the protease productive activity of S. speibonae TKU048 was optimal on 1% tuna head powder. Further, the casein/gelatin/tuna head powder zymography of the crude enzyme revealed the presence of three/nine/six proteases, respectively. The crude-enzyme cocktail of S. speibonae TKU048 exhibited the best proteolytic activity at 70 °C and pH = 5.8. Sodium dodecyl sulfate strongly enhanced the proteolytic activity of the cocktail, whereas FeCl3, CuSO4, and ethylenediaminetetraacetic acid could completely inhibit the enzyme activity. Additionally, the crude-enzyme cocktail of S. speibonae TKU048 could efficiently enhance the 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activities of all tested proteinaceous materials including the head, viscera, and meat of tuna fish; the head, viscera, and meat of tilapia fish; the head, meat, and shell of shrimp; squid pen; crab shell; and soybean. Taken together, S. speibonae TKU048 revealed potential in the reclamation of proteinaceous wastes for protease production and antioxidant preparation.
關鍵字 proteases;proteinaceous wastes;tuna head;Streptomyces speibonae;antioxidant
語言 en
ISSN 2410-3888
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 WANG San-Lang
審稿制度 是
國別 CHE
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/122773 )

SDGS 消除貧窮,消除飢餓,良好健康和福祉,潔淨水與衛生,可負擔的潔淨能源