Utilization of fishery-processing by-product squid pens for scale-up production of phenazines via microbial conversion and its novel potential antinematode effect
學年 110
學期 2
出版（發表）日期 2022-05-15
作品名稱 Utilization of fishery-processing by-product squid pens for scale-up production of phenazines via microbial conversion and its novel potential antinematode effect
作品名稱（其他語言）
著者 Nguyen TH; Wang SL; Nguyen TH; Doan MD; Tran THT; Ngo VA; Ho ND; Tran TN; Doan CT; Do VC; Nguyen AD; Nguyen VB
單位
出版者
著錄名稱、卷期、頁數 Fishes 7(3), 113
摘要 Fishery by-products (FBPs) have been increasingly investigated for the extraction and production of a vast array of active molecules. The aim of this study was to produce phenazine compounds from FBPs via microbial fermentation and assess their novel antinematode effect. Among various FBPs, squid pen powder (SPP) was discovered as the most suitable substrate for phenazine production by Pseudomonas aeruginosa TUN03 fermentation. Various small-scale experiments conducted in flasks for phenazine production indicated that the most suitable was the newly designed liquid medium which included 1% SPP, 0.05% MgSO4, and 0.1% Ca3(PO4)2 (initial pH 7). Phenazines were further studied for scale-up bioproduction in a 14 L bioreactor system resulting in a high yield (22.73 µg/mL) in a much shorter cultivation time (12 h). In the fermented culture broth, hemi-pyocyanin (HPC) was detected as a major phenazine compound with an area percentage of 11.28% in the crude sample. In the bioactivity tests, crude phenazines and HPC demonstrate novel potential nematicidal activity against black pepper nematodes, inhibiting both juveniles (J2) nematodes and egg hatching. The results of this work suggest a novel use of SPP for cost-effective bioproduction of HPC, a novel potential nematodes inhibitor. Moreover, the combination of MgSO4 and Ca3(PO4)2 was also found to be a novel salt composition that significantly enhanced phenazine yield by P. aeruginosa fermentation in this work.
關鍵字 fishery by-products;squid pens;bioconversion;phenazines;hemi-pyocyanin;black pepper nematodes
語言 en
ISSN 2410-3888
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 San-Lang, Wang
審稿制度 是
國別 CHE
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/122713 )

SDGS 消除貧窮,消除飢餓,良好健康和福祉,潔淨水與衛生,可負擔的潔淨能源