教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Production of thermophilic chitinase by Paenibacillus sp. TKU052 by bioprocessing of chitinous fishery wastes and its application in N-acetyl-D-glucosamine production
學年
學期
出版(發表)日期2021/09/09
作品名稱Production of thermophilic chitinase by Paenibacillus sp. TKU052 by bioprocessing of chitinous fishery wastes and its application in N-acetyl-D-glucosamine production
作品名稱(其他語言)
著者Doan CT; Tran TN; Wang SL
單位
出版者
著錄名稱、卷期、頁數Polymers 13(18), 3048
摘要The bioprocessing of chitinous fishery wastes (CFWs) to chitinases through fermentation
approaches has gained importance owing to its great benefits in reducing the enzyme production
cost, and utilizing chitin waste. In this work, our study of the chitinase production of Paenibacillus sp.
TKU052 in the presence of different kinds of CFWs revealed a preference for demineralized crab
shells powder (deCSP); furthermore, a 72 kDa chitinase was isolated from the 0.5% deCSP-containing
medium. The Paenibacillus sp. TKU052 chitinase displayed maximum activity at 70 ◦C and pH 4–5,
while Zn2+, Fe3+, Triton X-100, Tween 40, and SDS exerted a negative effect on its activity, whereas
Mn2+ and 2-mercaptoethanol were found to potentially enhance the activity. Among various kinds
of polysaccharide, Paenibacillus sp. TKU052 chitinase exhibited the best catalytic activity on colloidal
chitin (CC) with Km = 9.75 mg/mL and Vmax = 2.43 µmol/min. The assessment of the hydrolysis of
CC and N-acetyl chitooligosaccharides revealed that Paenibacillus sp. TKU052 chitinase possesses multiple catalytic functions, including exochitinase, endochitinase, and N-acetyl-β-D-glucosaminidase
activities. Finally, the combination of Paenibacillus sp. TKU052 chitinase and Streptomyces speibonae
TKU048 N-acetyl-β-D-glucosaminidase could efficiently convert CC to N-acetyl-D-glucosamine
(GlcNAc) with a production yield of 94.35–98.60% in 12–24 h.
關鍵字chitinous fishery wastes;chitinase;crab shells;Paenibacillus;N-acetyl-D-glucosamine
語言英文
ISSN2073-4360
期刊性質國內
收錄於SCI;
產學合作
通訊作者WANG San-Lang
審稿制度
國別中華民國
公開徵稿
出版型式,電子版
相關連結
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