Highly sensitive distance-based liquid crystalline visualization for paper-based analytical devices
學年 109
學期 2
出版(發表)日期 2021-02-18
作品名稱 Highly sensitive distance-based liquid crystalline visualization for paper-based analytical devices
作品名稱(其他語言)
著者 Khachornsakkul, K.; Chang, J.-J.; Lin, P.-H.; Lin, Y.-H.; Dungchai, W.; Chen, C.-H.
單位
出版者
著錄名稱、卷期、頁數 Analytica Chimica Acta 1154, 338328
摘要 We successfully report on the first demonstration of a highly sensitive distance-based liquid crystalline visualization for paper-based analytical devices. The construction of this paper sensor was achieved by immobilizing 4-cyano-4′-pentylbiphenyl (5CB) as liquid crystalline molecules (LCs) onto a paper strip substrate. The sensing mechanism is based on the ultrasound-assisted decomposition of 5CB by the hydroxyl radical (•OH) which is generated from the oxidase enzymatic reaction of the analyte, this then results in the change of texture and color of paper. The utility of our devices was then demonstrated with the determination of bilirubin (BR) in biological samples using a bilirubin oxidase enzymatic reaction. The quantification of BR can be achieved by dipping the tip of the paper strips into the analyte solutions and then by measuring the length of color which has been changed on the paper, by the naked eye. Under optimized conditions, this paper sensor offered the linear range of BR detection from 2.0 to 30.0 pmol/L (R2 = 0.9945) with the limit of detection (LOD) of 0.80 pmol/L. In addition, the results of this sensor were highly reproducible, with a relative standard deviation (RSD) of less than 3.50%. The recoveries of spiked BR in human urine and serum samples were in the range of 99.09–107.89%, which demonstrates the high accuracy of this paper sensor. Overall, this work presents a simple method to determine the concentration of H2O2 and BR at pmol levels with an instrument-free length-measuring readout, so it could be suitable for quantitative analysis of other biomarkers based on oxidase enzymatic reaction, which can provide important information about early disease diagnosis and patient prognosis.
關鍵字 Bilirubin;Liquid crystal;Ultrasound;Distance-based sensors;Paper-based sensors
語言 en_US
ISSN 1873-4324
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Chih-Hsin Chen
審稿制度
國別 NLD
公開徵稿
出版型式 ,電子版,紙本
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/120684 )