Bioprocessing of squid pens waste into chitosanase by Paenibacillus sp. TKU047 and its application in low-molecular weight chitosan oligosaccharides production
學年 108
學期 2
出版（發表）日期 2020-05-19
作品名稱 Bioprocessing of squid pens waste into chitosanase by Paenibacillus sp. TKU047 and its application in low-molecular weight chitosan oligosaccharides production
作品名稱（其他語言）
著者 Chien Thang Doan, Thi Ngoc Tran, Van Bon Nguyen, Trung Dung Tran, Anh Dzung Nguyen, San-Lang Wang
單位
出版者
著錄名稱、卷期、頁數 Polymers 12(5), 1163
摘要 Chitosan oligosaccharide (COS) has become of great interest in recent years because of its worthy biological activities. This study aims to produce COS using the enzymatic method, and investigates Paenibacillus sp. TKU047, a chitinolytic-producing strain, in terms of its chitosanase productivity on several chitinous material-containing mediums from fishery process wastes. The highest amount of chitosanase was produced on the medium using 2% (w/v) squid pens powder (0.60 U/mL) as the single carbon and nitrogen (C/N) source. The molecular mass of TKU047 chitosanase, which could be the smallest one among chitinases/chitosanases from the Paenibacillus genus, was approximately 23 kDa according to the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method. TKU047 chitosanase possessed the highest activity at 60 °C, pH 7, and toward chitosan solution with a higher degree of deacetylation (DDA) value. Additionally, the hydrolysis products of 98% DDA chitosan catalyzed by TKU047 chitosanase showed the degree of polymerization (DP) ranging from 2 to 9, suggesting that it was an endo-type activity chitosanase. The free radical scavenging activity of the obtained chitosan oligosaccharide (COS) was determined. The result showed that COS produced with Paenibacillus sp. TKU047 chitosanase expressed a higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity than that from the commercial COSs with maximum activity and IC50 values of 81.20% and 1.02 mg/mL; 18.63% and 15.37 mg/mL; and 15.96% and 15.16 mg/mL, respectively. As such, Paenibacillus sp. TKU047 may have potential use in converting squid pens waste to produce chitosanase as an enzyme for bio-activity COS preparation.
關鍵字 chitosan;chitosanase;chitosan oligosaccharides;Paenibacillus;free radical scavenging activity
語言 en
ISSN 2073-4360
期刊性質 國內
收錄於 SCI
產學合作
通訊作者 San-Lang Wang
審稿制度 是
國別 CHE
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/118704 )

SDGS 消除貧窮,永續城市與社區,消除飢餓,良好健康和福祉,可負擔的潔淨能源,尊嚴就業與經濟發展