Embryonic exposure to 4-methylimidazole leads to zebrafish myofibril misalignment
學年 107
學期 1
出版（發表）日期 2018-12-01
作品名稱 Embryonic exposure to 4-methylimidazole leads to zebrafish myofibril misalignment
作品名稱（其他語言）
著者 Jen‐Ning Tsai; Chiao‐Yin Sun; Yu‐Ju Ding; Yun‐Hsin Wang; Kang‐Chieh Lo Chi‐Chung Wen; Jia‐Wei Lin; Chiung‐Fang Chang; Li‐Sung Hsu; Hui‐Min Chen; Tsorng‐Harn Fong; Yau‐Hung Chen
單位
出版者
著錄名稱、卷期、頁數 Environmental Toxicology 33(12), p.1321-1328
摘要 4‐methylimidazole (4‐MI) is an imidazole‐derived organic chemical compound that can be used as a raw material in the manufacture of diverse chemicals and has been identified as an ingredient of caramel color in soybean sauce, beers, and other soft drinks. The aim of the present study was to investigate the teratogenic effects of 4‐MI during zebrafish embryogenesis. Zebrafish embryos were treated with different dosages of 4‐MI (0‐120 mM) for different exposure durations (12‐60 hours). The percentages of embryos with malformed phenotypes increased as the exposure dosages and duration time of 4‐MI increased. We also used immunofluorescence and transmission microscopy to evaluate the subtle changes in the myofibril alignment and ultrastructure of muscle organization. Our data showed that 4‐MI treatment disturbs muscle fiber alignment. Electron microscopy data indicated that Z‐lines were undetectable in the 4‐MI‐treated embryos. Although the thick and thin filaments were visible, they were all disorganized. In addition, zebrafish embryos treated by 4‐MI exhibited aberrant expression of 2 muscle‐specific genes, myod and myogenin. Taken together, we concluded that early exposure to 4‐MI affects zebrafish myogenesis, especially in myofibril alignment.
關鍵字 4‐methylimidazole;myogenesis;zebrafish
語言 en_US
ISSN
期刊性質 國外
收錄於 SCI SSCI
產學合作
通訊作者
審稿制度 否
國別 USA
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/117039 )