Study In-Plane Elasticity of Alveolar Epithelial Cells with Different Oncogene Expression Levels using Microfluidic Device
學年 106
學期 1
發表日期 2017-10-22
作品名稱 Study In-Plane Elasticity of Alveolar Epithelial Cells with Different Oncogene Expression Levels using Microfluidic Device
作品名稱（其他語言）
著者 Ko, P.-L.; Lee, T.-A.; Wang, C.-K.; Peng, C.-C.; Tung, Y.-C.
作品所屬單位
出版者
會議名稱 microTAS 2017
會議地點 Savannah, Georgia, USA
摘要 In this paper, we develop a microfluidic device with an embedded pressure sensor to study the in-plane direction elasticity of adenocarcinomic human alveolar epithelial (A549) cells with different oncogene, multiple copies in T-cell malignancy (MCT-1), expression levels [1]. The pressure sensor is constructed based on electrofluidic circuits, ionic liquid-filled microfluidic channel networks, with great long-term and temperature stabilities. The device consists of three polydimethylsiloxane (PDMS) layers: a top cell culture chamber layer, a middle sensing membrane layer and a bottom electrofluidic circuit layer as shown in Figure 1(a). On the top layer, a cell culture chamber with a single inlet and a single outlet is designed to culture cells for the measurement. On the bottom ionic liquid-filled circuit layer, four identical electrofluidic resistors [2] designed and arranged as a Wheatstone bridge circuit as shown in Figure 1(b). The membrane is sandwiched between the top and bottom layers. When the membrane is deformed by pressure application, the geometries of the electofluidic channel will be changed, and the characteristic of the electrofluidic circuit will also be changed accordingly. The change will further vary the output voltage signal from the circuit. When cells are seeded on the top of the sensing membrane, the cell- adhered membrane can be modeled as a two-layer composite plate. To quantitatively estimate the in-plane elasticity of a layer of cells, we derive a theoretical model based on first order shear deformation theory of plate [3-4] and basic circuit theories to estimate the cell elasticity from the sensor sensitivity variation. For comparison, we use an atomic force microscope (AFM) to measure the out-of-plane elasticity and thickness of the A549 cells. The average measured thickness of A549 cells is 1.11μm. With the measured pressure sensor output signals and the sensing membrane geometries and mechanical properties, we can calculate the relationship between the Young’s modulus of the cells layer and the sensitivity ratio. The ratio is obtained from the same device with and without the cells cultured in it (Figure 2). In the experiments, A549-control cells (A549-C) and A549 cells with MCT-1 oncogene overexpression (A549-M) [5] are used to investigate their in-plane elasticities. Figure 3 shows bright field phase images of the A549 cells cultured in the microfluidic devices during the experiments. Figure 4 (a) and (b) show the typical average sensitivity of the pressure sensor devices cultured A549-C and A549-M cells, respectively. According to the Figure 2, we can estimate the in-plane elasticity of A549-C and A540-M cells layers. Figure 5 shows comparison of the average in-plane elasticities of the A549-C and A549-M cells measured using the developed microfluidic devices. The results show that the average in-plane elasticities of A549-C and A549-M are 3.91 MPa and 8.74 MPa (n=3), respectively. The results demonstrate that the developed device can successfully measure the in-plane elasticity of the cells, and the in-plane elasticity increases when MCT-1 oncogene overexpressed in the A549 cells. With the demonstrated capability, the developed device shows its great potential for study of cell physical properties with different gene expression profiles.
關鍵字
語言 en
收錄於
會議性質 國際
校內研討會地點 無
研討會時間 20171022~20171026
通訊作者
國別 USA
公開徵稿
出版型式
出處 microTAS 2017
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/110306 )