| Immobilization of DNA on Microporous PVDF Membranes by Plasma Polymerization | |
|---|---|
| 學年 | 98 |
| 學期 | 1 |
| 出版（發表）日期 | 2009-12-01 |
| 作品名稱 | Immobilization of DNA on Microporous PVDF Membranes by Plasma Polymerization |
| 作品名稱（其他語言） | |
| 著者 | Lin, Dar-Jong; Lin, Dong-Tsamn; Young, Tai-Horng; Chen, Tsung-Chin; Chang, Hsu-Hsien; Cheng, Liao-Ping |
| 單位 | 淡江大學化學工程與材料工程學系 |
| 出版者 | Abingdon: Taylor & Francis Ltd. |
| 著錄名稱、卷期、頁數 | Journal of Biomaterials Science 20(13), pp.1943-1959 |
| 摘要 | Microporous poly(vinylidene fluoride) (PVDF) membranes with different porous surface morphologies were prepared by immersion-precipitation from coagulation baths of different strengths. On these membranes single-strand deoxyribonucleic acid (ss-DNA) was covalently immobilized by a dual-step procedure. First, poly(glycidyl methacrylate) (PGMA) was grafted on PVDF membranes by plasma-induced free radical polymerization. Then, ss-DNA was bonded to PGMA through ring-opening reactions of epoxies with amine to form amino alcohols. The highest attainable graft yield of PGMA on PVDF membrane was 0.3 mg/cm2, which was the case when a highly porous PVDF membrane was employed as the substrate. For immobilization of ss-DNA, the yield was found to depend significantly on the reaction temperature and pH. The maximal value was 48.5 μg/cm2. Furthermore, adsorption tests of anti-DNA antibody were carried out on membranes with and without immobilized ss-DNA using serum obtained from systemic lupus erythematosus patients. The results indicated that the immobilized DNA could effectively adsorb the antibody in the serum. |
| 關鍵字 | Poly(vinylidene fluoride) membrane; immobilization; DNA |
| 語言 | en |
| ISSN | 0920-5063 |
| 期刊性質 | 國外 |
| 收錄於 | SCI EI |
| 產學合作 | |
| 通訊作者 | Cheng, Liao-Ping |
| 審稿制度 | |
| 國別 | GBR |
| 公開徵稿 | |
| 出版型式 | 紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/53688 ) |
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