Immobilization of L-lysine on Microporous PVDF Membranes for Neuron Culture
學年 97
學期 2
出版（發表）日期 2009-03-01
作品名稱 Immobilization of L-lysine on Microporous PVDF Membranes for Neuron Culture
作品名稱（其他語言）
著者 Young, Tai-horng; Lin, Ui-hsiang; Lin, Dar-jong; Chang, Hsu-hsien; 鄭廖平; Cheng, Liao-ping
單位 淡江大學化學工程與材料工程學系
出版者 Brill Academic Publishers
著錄名稱、卷期、頁數 Journal of Biomaterials Science 20(5-6), pp.703-720
摘要 Microporous poly(vinylidene fluoride) (PVDF) membranes with dense or porous surface were prepared by immersion precipitation of PVDF/TEP solutions in coagulation baths containing different amounts of water. Onto the membrane surface, poly(glycidyl methacrylate) (PGMA) was grafted by plasma-induced free radical polymerization. Then, L-lysine was covalently bonded to the as-grafted PGMA through ring-opening reactions between epoxide and amine to form amino alcohol. The highest attainable graft density of PGMA on a PVDF membrane was 0.293 mg/cm<sup>2</sup>. This was obtained when the reaction was carried out on a porous surface under an optimized reaction condition. For immobilization of L-lysine, the yield was found to depend on the reaction temperature and L-lysine concentration. The maximal yield was 0.226 mg/cm<sup>2</sup>, a value considerably higher than reported in the literature using other immobilization methods. Furthermore, neurons were cultured on L-lysine-immobilized PVDF membranes. The results indicated that these membrane surfaces were suited to the growth of neurons, with a MTT value higher than that of the standard culture dish.
關鍵字 POLY(VINYLIDENE FLUORIDE);MEMBRANE;IMMOBILIZATION;L-LYSINE;NEURON;CULTURE
語言 en
ISSN 0920-5063
期刊性質 國內
收錄於
產學合作
通訊作者
審稿制度 否
國別 TWN
公開徵稿
出版型式 ,電子版
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