Immobilization of DNA on Microporous PVDF Membranes by Plasma Polymerization
學年 98
學期 1
出版（發表）日期 2009-12-01
作品名稱 Immobilization of DNA on Microporous PVDF Membranes by Plasma Polymerization
作品名稱（其他語言）
著者 Lin, Dar-Jong; Lin, Dong-Tsamn; Young, Tai-Horng; Chen, Tsung-Chin; Chang, Hsu-Hsien; Cheng, Liao-Ping
單位 淡江大學化學工程與材料工程學系
出版者 Abingdon: Taylor & Francis Ltd.
著錄名稱、卷期、頁數 Journal of Biomaterials Science 20(13), pp.1943-1959
摘要 Microporous poly(vinylidene fluoride) (PVDF) membranes with different porous surface morphologies were prepared by immersion-precipitation from coagulation baths of different strengths. On these membranes single-strand deoxyribonucleic acid (ss-DNA) was covalently immobilized by a dual-step procedure. First, poly(glycidyl methacrylate) (PGMA) was grafted on PVDF membranes by plasma-induced free radical polymerization. Then, ss-DNA was bonded to PGMA through ring-opening reactions of epoxies with amine to form amino alcohols. The highest attainable graft yield of PGMA on PVDF membrane was 0.3 mg/cm2, which was the case when a highly porous PVDF membrane was employed as the substrate. For immobilization of ss-DNA, the yield was found to depend significantly on the reaction temperature and pH. The maximal value was 48.5 μg/cm2. Furthermore, adsorption tests of anti-DNA antibody were carried out on membranes with and without immobilized ss-DNA using serum obtained from systemic lupus erythematosus patients. The results indicated that the immobilized DNA could effectively adsorb the antibody in the serum.
關鍵字 Poly(vinylidene fluoride) membrane; immobilization; DNA
語言 en
ISSN 0920-5063
期刊性質 國外
收錄於 SCI EI
產學合作
通訊作者 Cheng, Liao-Ping
審稿制度
國別 GBR
公開徵稿
出版型式 紙本
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