An Amperometric Urea Bisosensor based on Covalent Immobilization of Urease on N2 Incorporated Diamond Nanowire Electrode
學年 102
學期 2
出版（發表）日期 2014-06-01
作品名稱 An Amperometric Urea Bisosensor based on Covalent Immobilization of Urease on N2 Incorporated Diamond Nanowire Electrode
作品名稱（其他語言）
著者 Jayakumar Shalini; Kamatchi Jothiramalingam Sankaran; Lee, Chi-Young; Tai, Nyan-Hwa; Lin, I-Nan
單位 淡江大學物理學系
出版者 Amsterdam: Elsevier BV
著錄名稱、卷期、頁數 Biosensors and Bioelectronics 56, pp.64–70
摘要 N2 incorporated diamond nanowire (N-DNW) film electrochemical biosensor has utilized for the quantitative determination of urea in aqueous solution and urine sample. N-DNW electrode is wet-chemically cleaned (oxidation) by boiling in a mixture of H2SO4 and HNO3 (3:1) at 200 °C for 2 h to remove graphite. Urease (Urs) and glutamate dehydrogenase (GLDH) are covalently attached to the oxidized N-DNW electrode by activating the COOH group of N-DNW using ethyl(dimethylaminopropyl)carbodiimide as the coupling agent and N-hydroxysuccinimide as activator. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy data reveal that carboxylic and hydroxyl functionalized nature of N-DNW electrodes Urs–GLDH immobilized N-DNW (Urs–GLDH/N-DNW) has been successfully utilized in urea biosensor which exhibits good performance in sensitivity (6.18 μA/mg dL/cm2), stability (~1 month), reproducibility, lower detection limit (3.87 mg/dL) and fast response time (>10 s). Urs–GLDH/N-DNW also exhibits electrochemical response when tested for different concentration of human urine in buffer solution (from 1:9 to 4:6). In addition, Urs–GLDH/N-DNW bioelectrode retains 80% of its initial enzyme activity for <1 month, when stored at 4–6 °C in a refrigerator.
關鍵字 N2 incorporated diamond nanowire; Urs–GLDH/N-DNW; Urea; Cyclic voltammetry; Electrochemical impedance spectrsocopy; Amperometry
語言 en
ISSN 0956-5663
期刊性質
收錄於
產學合作
通訊作者 Lee, Chi-Young
審稿制度
國別 NLD
公開徵稿
出版型式 紙本
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