Rhodamine-ethylenediol, a novel vital fluorescent probe for labeling alkaline phosphatase-rich organelles
學年 99
學期 1
出版（發表）日期 2010-12-01
作品名稱 Rhodamine-ethylenediol, a novel vital fluorescent probe for labeling alkaline phosphatase-rich organelles
作品名稱（其他語言）
著者 Chen, Yau-hung; Chiou, Chien-hui; Chen, Wei-li; Jhou, Yu-ru; Lee, Ya-ting; Cheng, Chien-chung
單位 淡江大學化學學系; 淡江大學企業管理學系
出版者 臺北市：中國化學學會
著錄名稱、卷期、頁數 Journal of the Chinese Chemical Society=中國化學會會誌 57(6), pp.1257-1261
摘要 Zebrafish have received considerable attention as an organism-based model in the development of pharmacological agents. Many small moleculesapplied to zebrafish show important behaviours and may constitute new kinds of markers for clinical purposes. Analysis of these molecules can facilitate the development of useful tools for monitoring environmental changes. Many chemicals that are toxic to the environment are known to influence the sensory systems of humans and fish. One important sensory system in all fish is the lateral line organ, which is readily accessible for the assessment of environmental changes. Neuromasts, which are located on the surface of the fish body, are one of the major components of the lateral lines of the zebrafish. Copper enriched water is known to affect the olfactory system in fish. Therefore, small molecules that induce specific patterns in the neuromasts of zebrafish should provide an important animal model with which to explore the effects of environmental changes on the sensory system.Recently, chemical sensors based on the rhodamine skeleton have been designed to specifically detect metal ions, such as Cu(II) and Fe(III)/Hg(II), in zebrafish. However, there has been no report of these rhodamine derivatives used in the specific recognition of the sensory system of zebrafish. Commonly, the sensory system is studied with antibody staining assays of scarified fish. Here, we report that a new rhodamine derivative can be used as a fluorescent chemical probe to visualize the neuromasts and intestinal villi of living zebrafish. Based on the specific recognition of this area in zebrafish, we narrowed the possible enzymes targeted by this rhodamine probe to alkaline phosphatase and confirmed this with a binding assay. It is a well-recognized challenge to develop a fluorescent chemical probe that specifically recognizes a particular enzyme. Furthermore, the transfer of phosphate groups to certain enzymes can activate their catalytic reactivity, triggering a cascade reaction in a signal transduction pathway. The alkaline phosphatase specific recognition by this rhodamine derivative may be applicable to clinical purposes.
關鍵字
語言 en
ISSN 0009-4536
期刊性質 國內
收錄於 SCI
產學合作
通訊作者 Chen, Yau-hung
審稿制度 否
國別 TWN
公開徵稿
出版型式 ,紙本
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