Integrating SPR-ellipsometry and electrochemical measurements for performance evaluation of label-free thiophene-based biosensor
學年 102
學期 2
出版（發表）日期 2014-05-01
作品名稱 Integrating SPR-ellipsometry and electrochemical measurements for performance evaluation of label-free thiophene-based biosensor
作品名稱（其他語言）
著者 Tsai, Pei-I; Lee, Shu-Sheng; Chou, Shin-Ting; Chang, Yu-Ting; Lee, Adam Shih-Yuan; Lee, C.-K.
單位 淡江大學化學學系; 台灣大學應用力學研究所
出版者 S P I E-International Society for Optical Engineering
著錄名稱、卷期、頁數 Proceedings of SPIE 8933, pp.89330H
摘要 The surface plasmon resonance reflectance changes measured with a circularly polarized ellipsometry and an electrochemical impedance spectroscopy were identified to be able to characterize the critical roles of biomolecules for vastly different biological functions and processes. Throughout the course of this study, interferon-gamma (IFN-γ) was chosen as the biomarker to test and to verify the performance of this newly developed system for Tuberculosis detection. The interactions of IFN-γ with immobilized anti-IFN-γ antibody at various concentrations were interrogated both optically and electrochemically. A semi-conductive linker bis-thiophene was thiolated to ensure the cross-linked monoclonal human IFN-γ antibody got self-assembled onto the gold thin film and form a label-free biosensor. The functional features of the bis-thiophene coated-gold film were characterized by cyclic voltammetry and impedance spectroscopy methods. The association of IFN-γ to the bis-thiophene bridging units via antibody-antigen interactions provided the basis for ultrasensitive detection of IFN-γ by tracking the conformation changes in surface-bound protein molecules. The phase shift can be attributed to the average thickness and the real-time index of refraction of the protein layer in different protein layer. Experimental results obtained by impedance spectroscopy and by phase-interrogation SPR showed linear dynamic range. Our experimental results verified that an increase in the concentration of the IFN-γ usually accompanied by phase increase in SPR and an impedance decrease in EIS. These results indicated that our newly developed integrated biosensing system can potentially provide new insight into various conjugate phenomena and interfacial processes for observing molecular conformation changes.
關鍵字 Ellipsometry; Biosensors; Dielectric spectroscopy; Gold; Molecules; Phase shifts; Refractive index; Thin films; Biosensing
語言 en
ISSN 0277-786X 1996-756X
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Lee, C.-K.
審稿制度 是
國別 USA
公開徵稿
出版型式 電子版 紙本
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