Tyrosinase inhibitory activity of supernatant and semi-purified extracts from squid pen fermented with Burkholderia cepacia TKU025
學年 102
學期 2
出版（發表）日期 2014-06-22
作品名稱 Tyrosinase inhibitory activity of supernatant and semi-purified extracts from squid pen fermented with Burkholderia cepacia TKU025
作品名稱（其他語言）
著者 Liang, Tzu-Wen
單位 淡江大學化學學系
出版者 Dordrecht: Springer Netherlands
著錄名稱、卷期、頁數 Research on Chemical Intermediates 41,　p.6105-6116
摘要 Reports of tyrosinase inhibitors from microorganisms are rare. The tyrosinase inhibitory activity was found from the culture supernatant of Burkholderia cepacia TKU025 with 1 % squid pen as the sole carbon/nitrogen source. The fourth-day supernatant showed the strongest tyrosinase inhibitory activity (500 U/ml) and the highest total phenolic content (789 μg/ml GAE). The supernatant was further purified by liquid–liquid partition, and it was found that the methanol extract exhibited the lowest IC50 value of 2 μg/ml in the tyrosinase inhibitory activity assay and was compared with the positive control kojic acid and vitamin C. Significant associations between the tyrosinase inhibitory potency and the total phenolic content were found for the supernatant, extract, and fractions. The tyrosinase inhibitory activity persisted even when it was treated with acidic or alkaline conditions of pH 2 or 12. The tyrosinase inhibitory activity remained at 100 %, even after treatment at 100 °C for 60 min. The tyrosinase inhibitor also showed antimicrobial activity against Escherichia coli, Bacillus cereus, Aspergillus fumigatus, and Fusarium oxysporum.
關鍵字 Tyrosinase inhibitor;Phenolic content;Squid pen;Antibacterial;Antifungal;Burkholderia cepacia
語言 en
ISSN 1568-5675
期刊性質 國外
收錄於
產學合作
通訊作者 Wang, San-Lang
審稿制度 否
國別 NLD
公開徵稿
出版型式 ,電子版,紙本
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