| Resveratrol treatment attenuates the wound-induced inflammation in zebrafish larvae through the suppression of myeloperoxidase expression. | |
|---|---|
| 學年 | 99 |
| 學期 | 2 |
| 出版（發表）日期 | 2011-06-01 |
| 作品名稱 | Resveratrol treatment attenuates the wound-induced inflammation in zebrafish larvae through the suppression of myeloperoxidase expression. |
| 作品名稱（其他語言） | |
| 著者 | Liao, Y.F.; Chiou, M.C.; Tsai, J.N.; Wen, C.C.; Wang, Y.H.; Cheng, C.C.; Chen, Y.H. |
| 單位 | 淡江大學數學學系暨研究所 |
| 出版者 | Taipei: Bureau of Food and Drug Analysis |
| 著錄名稱、卷期、頁數 | Journal of Food and Drug Analysis 19(2), pp.167-173 |
| 摘要 | Resveratrol, a polyphenolic phytoalexin found in many plants, was reported to exhibit anti-inflammatory effects, but its molecular mechanism is not fully understood. This study was aimed to investigate the anti-inflammatory effects of resveratrol in vivo using a zebrafish model of wound-induced inflammation. Caudal fin-wounded zebrafish larvae were treated with resveratrol for 8 h. Neutrophil recruitment was visualized in transgenic line "Tg (mpx : GFP)" expressing GFP-tagged neutrophil-specific myeloperoxidase (mpx). The enzymatic activity of Mpx was evaluated by histochemical staining. Relative mRNA levels of mpx and cyclooxegenase-2 (cox2) were quantified by qRT-PCR, and the protein expression levels of Mpx and Cox2 were detected by immunostaining. Results showed that wound-induced neutrophil recruitment in zebrafish was not affected by resveratrol, but Mpx enzymatic activity in zebrafish was significantly suppressed by resveratrol in a dose-dependent manner. Moreover, both mRNA and protein expression levels of Mpx and Cox2 were significantly down-regulated by resveratrol. Taken together, our results provide in vivo evidence that the anti-inflammatory effects of resveratrol on wound-induced inflammation are significantly mediated through the suppression of Mpx and Cox2 at both transcriptional and protein levels, rather than the down-regulation of neutrophil recruitment. In conclusion, this in vivo zebrafish model can be readily applied to screen other potential anti-inflammatory compounds at a whole-organism level. |
| 關鍵字 | cyclooxygenase; inflammation; myeloperoxidase; resveratrol; zebrafish |
| 語言 | en_US |
| ISSN | 1021-9498 |
| 期刊性質 | 國際 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 是 |
| 國別 | TWN |
| 公開徵稿 | |
| 出版型式 | 紙本 電子版 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/97764 ) |
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