Resveratrol treatment attenuates the wound-induced inflammation in zebrafish larvae through the suppression of myeloperoxidase expression.
學年 99
學期 2
出版（發表）日期 2011-06-01
作品名稱 Resveratrol treatment attenuates the wound-induced inflammation in zebrafish larvae through the suppression of myeloperoxidase expression.
作品名稱（其他語言）
著者 Liao, Y.F.; Chiou, M.C.; Tsai, J.N.; Wen, C.C.; Wang, Y.H.; Cheng, C.C.; Chen, Y.H.
單位 淡江大學數學學系暨研究所
出版者 Taipei: Bureau of Food and Drug Analysis
著錄名稱、卷期、頁數 Journal of Food and Drug Analysis 19(2), pp.167-173
摘要 Resveratrol, a polyphenolic phytoalexin found in many plants, was reported to exhibit anti-inflammatory effects, but its molecular mechanism is not fully understood. This study was aimed to investigate the anti-inflammatory effects of resveratrol in vivo using a zebrafish model of wound-induced inflammation. Caudal fin-wounded zebrafish larvae were treated with resveratrol for 8 h. Neutrophil recruitment was visualized in transgenic line "Tg (mpx : GFP)" expressing GFP-tagged neutrophil-specific myeloperoxidase (mpx). The enzymatic activity of Mpx was evaluated by histochemical staining. Relative mRNA levels of mpx and cyclooxegenase-2 (cox2) were quantified by qRT-PCR, and the protein expression levels of Mpx and Cox2 were detected by immunostaining. Results showed that wound-induced neutrophil recruitment in zebrafish was not affected by resveratrol, but Mpx enzymatic activity in zebrafish was significantly suppressed by resveratrol in a dose-dependent manner. Moreover, both mRNA and protein expression levels of Mpx and Cox2 were significantly down-regulated by resveratrol. Taken together, our results provide in vivo evidence that the anti-inflammatory effects of resveratrol on wound-induced inflammation are significantly mediated through the suppression of Mpx and Cox2 at both transcriptional and protein levels, rather than the down-regulation of neutrophil recruitment. In conclusion, this in vivo zebrafish model can be readily applied to screen other potential anti-inflammatory compounds at a whole-organism level.
關鍵字 cyclooxygenase; inflammation; myeloperoxidase; resveratrol; zebrafish
語言 en_US
ISSN 1021-9498
期刊性質 國際
收錄於 SCI
產學合作
通訊作者
審稿制度 是
國別 TWN
公開徵稿
出版型式 紙本 電子版
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