Caffeine treatment disturbs the angiogenesis of zebrafish embryos
學年 101
學期 1
出版（發表）日期 2012-10-01
作品名稱 Caffeine treatment disturbs the angiogenesis of zebrafish embryos
作品名稱（其他語言）
著者 Yeh, C.H.; Liao, Y.F.; Chang, C.Y.; Tsai, J.N.; Wang, Y.H.; Cheng, C.C.; Wen, C.C.; Chen, Y.H.
單位 淡江大學數學學系暨研究所
出版者 New York: Informa Healthcare
著錄名稱、卷期、頁數 Drug and Chemical Toxicology 35(4), pp.361-365
摘要 Caffeine is a widely consumed substance that occurs in numerous dietary sources, but teratogenic effects of caffeine intake during embryonic development are still not clear. In the present study, we used the zebrafish as a model to assess caffeine-induced toxicity on embryonic vascular development. A green fluorescent vascular endothelium transgenic line, Tg(fli1:egfp), was utilized for the sensitive detection of vascular development, including vasculo- and angiogenesis. Caffeine-treated embryos showed no defects in vasculogenesis, but revealed dose-dependent (250-350 ppm) developmental defects in intersegmental vessels, dorsal longitudinal anastomotic vessels, and subintestinal vein sprouting. Further, real-time polymerase chain reaction analysis of caffeine-treated embryos showed an upregulation of nrp1a along with a downregulation of sema3aa and sema3c. In conclusion, caffeine treatment induces defects of angiogenesis in zebrafish embryos.
關鍵字 Angiogenesis;caffeine;fluorescent protein;transgenic fish;zebrafish
語言 en_US
ISSN 0148-0545
期刊性質 國外
收錄於 SCI
產學合作 國外
通訊作者
審稿制度 否
國別 USA
公開徵稿
出版型式 ,電子版,紙本
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