Transgenic expression of prothymosin alpha on zebrafish epidermal cells promotes proliferation and attenuates UVB-induced apoptosis
學年 99
學期 1
出版（發表）日期 2010-08-01
作品名稱 Transgenic expression of prothymosin alpha on zebrafish epidermal cells promotes proliferation and attenuates UVB-induced apoptosis
作品名稱（其他語言）
著者 Pai, Chiung-Wen; Chen, Yau-Hung
單位 淡江大學化學學系
出版者 Dordrecht: Springer Netherlands
著錄名稱、卷期、頁數 Transgenic Research 19(4), pp.655-665
摘要 This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. However, no evident morphological abnormalities were observed. Thus, overexpression of Ptmaa alone is not sufficient to cause disorganized growths or even cancer in zebrafish skin. Molecular and histological evidences showed that Tg(k18:Ptmaa-RFP) embryos have more proliferating cells in the pelvic fins [WT: 3.92 ± 7.15; Tg(k18:Ptmaa-RFP): 38.00 ± 10.87] and thicker skin [WT: 10.98 ± 1.41 μm; Tg(k18:Ptmaa-RFP): 14.02 ± 1.32 μm], indicating that overexpression of Ptmaa can promote proliferation. On the other hand, fewer apoptotic signals were found when Tg(k18:Ptmaa-RFP) embryos were exposed to UVB. Together with quantitative RT-PCR data, we suggest that UVB-induced epidermal cell apoptosis of zebrafish larvae can be attenuated by overexpression of Ptmaa through the enhancement of transcriptions of bcl2 mRNAs. Taken together, we conclude that overexpression of Ptmaa in zebrafish epidermal cells promotes proliferation and attenuates UVB-induced apoptosis but does not cause skin cancer.
關鍵字
語言 en
ISSN 0962-8819; 1573-9368
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Chen, Yau-Hung
審稿制度 否
國別 NLD
公開徵稿
出版型式 ,電子版,紙本
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