Endo-β-N-acetylglucosaminidase from fig latex
學年 65
學期 1
出版(發表)日期 1977-01-01
作品名稱 Endo-β-N-acetylglucosaminidase from fig latex
作品名稱(其他語言)
著者 Chien, Su-Fang; Weinburg, Robin; Li, Su-Chen; Li, Yu-Teh
單位 淡江大學化學學系
出版者 Maryland Heights: Academic Press
著錄名稱、卷期、頁數 Biochemical and Biophysical Research Communications 76(2), pp.317-323
摘要 Commercially available fig latex contains several endo-β-N-acetylglucosaminidases which catalyze the reaction: (Man)nG1cNAcβ1→4G1cNAcAsn → (Man)nG1cNAc + G1cNAcAsn. Using (NH4)2SO4 fractionation followed by chromatography on Sephadex G-100 and DEAE-Sephadex A-50, two distinct types of endo-β-N-acetylglucosaminidases have been partially purified and characterized. One, called F-I, hydrolyzes the di-N-acetylchitobiosyl linkage in the glycopeptide, (Man)3(G1cNAc)2Asn prepared from human IgG, much faster than that linkage in the glycopeptides, (Man)5(G1cNAc)2Asn and (Man)6(G1cNAc)2Asn both from ovalbumin. The other, called F-II, hydrolyzes the same linkage in (Man)5(G1cNAc)2-Asn and (Man)6(G1cNAc)2Asn, but not that in (Man)3(G1cNAc)2Asn.
關鍵字
語言 en
ISSN 0006-291X
期刊性質 國外
收錄於
產學合作
通訊作者 Li, Y.T.
審稿制度
國別 USA
公開徵稿
出版型式 紙本
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