| A Liquid Crystal Biosensor for Detecting Organophosphates through the Localized pH Changes Induced by Their Hydrolytic Products | |
|---|---|
| 學年 | 101 |
| 學期 | 2 |
| 出版（發表）日期 | 2013-05-01 |
| 作品名稱 | A Liquid Crystal Biosensor for Detecting Organophosphates through the Localized pH Changes Induced by Their Hydrolytic Products |
| 作品名稱（其他語言） | |
| 著者 | Chen, Chih-hsin; Yang, Kun-lin |
| 單位 | 淡江大學化學學系 |
| 出版者 | Lausanne: Elsevier S.A. |
| 著錄名稱、卷期、頁數 | Sensors and Actuators B: Chemical 181, pp.368-374 |
| 摘要 | We report a biosensor for detecting organophosphates (OPs) by using liquid crystal (LC). The mechanism of the biosensor is based on detection of minute pH changes during the enzymatic hydrolysis of OPs. To hydrolyze OPs, paraoxonase 1 (PON1) is immobilized on a copper grid which also hosts pH-sensitive LC, 4-cyano-4′-pentylbiphenyl (5CB) doped with 0.3% of 4′-pentyl-biphenyl-4-carboxylic acid (PBA). Proximity of enzyme and LC ensures that H+ can be detected by the pH-sensitive LC before it is neutralized by buffer. Thus, the presence of OPs can be reported as color change in LC when they undergo enzymatic hydrolysis. For paraoxon detection, we can reach a limit of detection (LOD) of 1 μM by using the naked eye. This type of LC-based biosensor also shows high specificity and does not respond to imidacloprid and ampicillin. |
| 關鍵字 | Organophosphate;Biosensors;Liquid crystal;Paraoxonase 1 |
| 語言 | en |
| ISSN | 0925-4005;1873-3077 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Yang, Kun-lin |
| 審稿制度 | 是 |
| 國別 | NLD |
| 公開徵稿 | |
| 出版型式 | ,電子版,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/89108 ) |
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