A Liquid Crystal Biosensor for Detecting Organophosphates through the Localized pH Changes Induced by Their Hydrolytic Products
學年 101
學期 2
出版(發表)日期 2013-05-01
作品名稱 A Liquid Crystal Biosensor for Detecting Organophosphates through the Localized pH Changes Induced by Their Hydrolytic Products
作品名稱(其他語言)
著者 Chen, Chih-hsin; Yang, Kun-lin
單位 淡江大學化學學系
出版者 Lausanne: Elsevier S.A.
著錄名稱、卷期、頁數 Sensors and Actuators B: Chemical 181, pp.368-374
摘要 We report a biosensor for detecting organophosphates (OPs) by using liquid crystal (LC). The mechanism of the biosensor is based on detection of minute pH changes during the enzymatic hydrolysis of OPs. To hydrolyze OPs, paraoxonase 1 (PON1) is immobilized on a copper grid which also hosts pH-sensitive LC, 4-cyano-4′-pentylbiphenyl (5CB) doped with 0.3% of 4′-pentyl-biphenyl-4-carboxylic acid (PBA). Proximity of enzyme and LC ensures that H+ can be detected by the pH-sensitive LC before it is neutralized by buffer. Thus, the presence of OPs can be reported as color change in LC when they undergo enzymatic hydrolysis. For paraoxon detection, we can reach a limit of detection (LOD) of 1 μM by using the naked eye. This type of LC-based biosensor also shows high specificity and does not respond to imidacloprid and ampicillin.
關鍵字 Organophosphate;Biosensors;Liquid crystal;Paraoxonase 1
語言 en
ISSN 0925-4005;1873-3077
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Yang, Kun-lin
審稿制度
國別 NLD
公開徵稿
出版型式 ,電子版,紙本
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