| 標題：Biodegradation of shellfish wastes and production of chitosanases by a squid pen-assimilating bacterium, Acinetobacter calcoaceticus TKU024 |
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| 學年 | 100 |
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| 學期 | 1 |
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| 出版（發表）日期 | 2011/09/01 |
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| 作品名稱 | Biodegradation of shellfish wastes and production of chitosanases by a squid pen-assimilating bacterium, Acinetobacter calcoaceticus TKU024 |
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| 作品名稱（其他語言） | |
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| 著者 | Wang, San-Lang; Tseng, Wan-Nine; Liang, Tzu-Wen |
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| 單位 | 淡江大學生命科學研究所; 淡江大學化學學系 |
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| 出版者 | Dordrecht: Springer Netherlands |
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| 著錄名稱、卷期、頁數 | Biodegradation 22(5), pp.939-948 |
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| 摘要 | Two chitosanases (CHSA1 and CHSA2) were purified from the culture supernatant of Acinetobacter calcoaceticus TKU024 with squid pen as the sole carbon/nitrogen source. The molecular masses of CHSA1 and CHSA2 determined by SDS-PAGE were approximately 27 and 66 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of CHSA1 and CHSA2 were (pH 6, 50°C, pH 4–10, ＜90°C) and (pH 7, 60°C, pH 6–11, ＜70°C), respectively. CHSA1 and CHSA2 had broad pH and thermal stability. CHSA1 and CHSA2 were both inhibited by EDTA and were inhibited completely by 5 mM Mn2+. CHSA1 and CHSA2 degraded chitosan with DD ranging from 60 to 98%, and also degraded some chitin. The most susceptible substrate was 60% deacetylated chitosan. Furthermore, TKU024 culture supernatant (1.5% SPP) incubated for 5 days has the most reducing sugars (0.63 mg/ml). With this method, we have shown that shellfish wastes may have a great potential for the production of bioactive materials. |
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| 關鍵字 | Chitosanase;Squid pen wastes;Acinetobacter calcoaceticus;Reducing sugar |
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| 語言 | 英文(美國) |
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| ISSN | 1572-9729;0923-9820 |
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| 期刊性質 | 國外 |
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| 收錄於 | SCI |
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| 產學合作 | |
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| 通訊作者 | Wang, San-Lang |
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| 審稿制度 | 是 |
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| 國別 | |
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| 公開徵稿 | |
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| 出版型式 | 電子版;紙本 |
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| 相關連結 | |
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| SDGs | |
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