Fermented and enzymatic production of chitin/chitosan oligosaccharides by extracellular chitinases from Bacillus cereus TKU027
學年 101
學期 1
出版（發表）日期 2012-10-15
作品名稱 Fermented and enzymatic production of chitin/chitosan oligosaccharides by extracellular chitinases from Bacillus cereus TKU027
作品名稱（其他語言）
著者 Wang, San-Lang; Liu, Chin-Pei; Liang, Tzu-Wen
單位 淡江大學生命科學研究所; 淡江大學化學學系
出版者 Kidlington: Pergamon
著錄名稱、卷期、頁數 Carbohydrate Polymers 90(3), pp.1305–1313
摘要 Two chitinases, Chi I and Chi II, were purified from the culture supernatant of Bacillus cereus TKU027 with shrimp head powder (SHP) as the sole carbon/nitrogen source. The molecular masses of Chi I and Chi II determined using SDS–PAGE were approximately 65 kDa and 63 kDa, respectively. Chi I toward various surfactants showed high stability, such as SDS, Tween 20, Tween 40 and Triton X-100, and these surfactants were stimulator of Chi I chitinase activity. Concomitant with the production of Chi I and Chi II, chitin oligosaccharides were also observed in the culture supernatant, including chitobiose, chitotriose, chitotetrose and chitopentose at concentrations of 0.44 mg/mL, 0.08 mg/mL, 0.09 mg/mL and 0.43 mg/mL, respectively. Chitosan with 60% deacetylation was degraded by TKU027 crude enzyme to prepare chitooligosaccharides. MALDI-TOF MS analysis of the enzymatic hydrolyzates indicated that the products were mainly chitooligosaccharides with degree of polymerization (DP) in the 4–9 range.
關鍵字 Chitinase;Chitin oligosaccharides;Ahrimp heads;Bacillus cereus;Fermented;Enzymatic hydrolysis
語言 en
ISSN 1879-1344
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 王三郎
審稿制度 是
國別 GBR
公開徵稿
出版型式 ,電子版,紙本
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