教師資料查詢 | 類別: 期刊論文 | 教師: 林孟山 Lin, Meng-shan (瀏覽個人網頁)

標題:Amperometric determination of NADH with Co3O4 nanosheet modified electrode
學年
學期
出版(發表)日期2013/04/01
作品名稱Amperometric determination of NADH with Co3O4 nanosheet modified electrode
作品名稱(其他語言)
著者Chen, Chi-Hao; Chen, Ying-Cih; Lin, Meng-Shan
單位淡江大學化學學系
出版者Amsterdam: Elsevier BV
著錄名稱、卷期、頁數Biosensors and Bioelectronics 42, pp.379-384
摘要In this work, we have developed a simple and reliable cobalt oxide (Co3O4) based amperometric sensor for the determination of NADH. A sheet shape Co3O4 nanooxide was synthesized by the CTAB assisted hydrothermal technique and was characterized by SEM and XPS. Owing to the redox property of Co3O4, the operating potential of NADH can be significantly reduced from 0.7 down to 0.1 V. Compared to a commercial Co3O4 nanoparticle modified electrode, this nanosheet form cobalt oxide possesses a rapid background subsiding characteristic and a low residual current. This scheme was conducted on a flow injection system with a constant operating potential of 0.1 V (vs. Ag/AgCl, 3 M) in a 0.2 M phosphate buffer at pH 6.0. A suitable linear range from 10 to 100 μM (R=0.999) with a detection limit of 4.25 μM (S/N=3) was obtained. The RSD for 20 successive measurements of 75 μM NADH is only 1.4%, which indicates a high stability and no contamination during NADH oxidation. This scheme did not suffer from conventional antioxidants, including dopamine, uric acid, epinephrine, serotonin, histamine, and 4-acetaminophen, except ascorbic acid. Thus, an ascorbate oxidase was introduced to remove the ascorbic acid before the sample was injected into the flow injection analysis system. After this simple pretreatment, the influence of ascorbic acid was eliminated, successfully.
關鍵字NADH; Metal oxide; Cobalt oxide; Amperometry
語言英文
ISSN0956-5663;1873-4235
期刊性質國外
收錄於SCI;
產學合作
通訊作者Lin, Meng-Shan
審稿制度
國別荷蘭
公開徵稿
出版型式,電子版,紙本
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