教師資料查詢 | 類別: 期刊論文 | 教師: 簡素芳CHIEN, SU-FENG (瀏覽個人網頁)

標題:Taro α-galactosidase: A new gene product for blood conversion
學年100
學期2
出版(發表)日期2012/04/01
作品名稱Taro α-galactosidase: A new gene product for blood conversion
作品名稱(其他語言)
著者Chern, Ming-kai; Li, Huang-yi; Chen, Po-fan; Chien, Su-fang
單位淡江大學化學學系; 淡江大學生命科學研究所
出版者Amsterdam: Elsevier BV
著錄名稱、卷期、頁數Biocatalysis and Agricultural Biotechnology 1(2),pp.135-139
摘要The N-terminal sequence and internal sequences of the purified taro α-galactosidase (α-Gal) were determined using tandem mass spectrometry. By using reverse transcriptase PCR (RT-PCR), 5′ and 3′ rapid amplification of cDNA ends (RACE) with designed, degenerate primers, a novel cDNA sequence was obtained. The recombinant taro α-Gal not only hydrolyzes α1→4 linked galactosyl residues, which are accumulated in the tissues from patients with Fabry disease, but also hydrolyzes the α1→3 linked galactoside of B red blood cells (RBC). The recombinant taro α-Gal provides an ideal enzyme source for biomedical systems.
關鍵字Taro α-galactosidase;Colocasia esculenta;Pichia pastoris expression;Mass spectrometry;Red blood cell
語言英文
ISSN1878-8181
期刊性質國外
收錄於
產學合作
通訊作者Chern, Ming-kai; Chien, Su-fang
審稿制度
國別荷蘭
公開徵稿
出版型式電子版
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