Taro α-galactosidase: A new gene product for blood conversion
學年 100
學期 2
出版(發表)日期 2012-04-01
作品名稱 Taro α-galactosidase: A new gene product for blood conversion
著者 Chern, Ming-kai; Li, Huang-yi; Chen, Po-fan; Chien, Su-fang
單位 淡江大學化學學系; 淡江大學生命科學研究所
出版者 Amsterdam: Elsevier BV
著錄名稱、卷期、頁數 Biocatalysis and Agricultural Biotechnology 1(2),pp.135-139
摘要 The N-terminal sequence and internal sequences of the purified taro α-galactosidase (α-Gal) were determined using tandem mass spectrometry. By using reverse transcriptase PCR (RT-PCR), 5′ and 3′ rapid amplification of cDNA ends (RACE) with designed, degenerate primers, a novel cDNA sequence was obtained. The recombinant taro α-Gal not only hydrolyzes α1→4 linked galactosyl residues, which are accumulated in the tissues from patients with Fabry disease, but also hydrolyzes the α1→3 linked galactoside of B red blood cells (RBC). The recombinant taro α-Gal provides an ideal enzyme source for biomedical systems.
關鍵字 Taro α-galactosidase;Colocasia esculenta;Pichia pastoris expression;Mass spectrometry;Red blood cell
語言 en
ISSN 1878-8181
期刊性質 國外
通訊作者 Chern, Ming-kai; Chien, Su-fang
國別 NLD
出版型式 電子版

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/76985 )