Electroporation for three commonly used yeast strains for two-hybrid screening experiments
學年 100
學期 1
出版(發表)日期 2011-09-01
作品名稱 Electroporation for three commonly used yeast strains for two-hybrid screening experiments
作品名稱(其他語言)
著者 Lin, Hsin-ying; Lin, Sey-en; Chien, Su-fang; Chern, Ming-kai
單位 淡江大學化學學系
出版者 Philadelphia: Elsevier Inc.
著錄名稱、卷期、頁數 Analytical Biochemistry 416(1), pp.117-119
摘要 The efficiency of transformation by electroporation has been known to be compromised by strain dependency. A high efficiency protocol is still lacking for distinct two-hybrid yeast strains of diverse genetic features. Here, we used 0.5 M lithium acetate (LiAc) and 50 mM Tris-HCl with 5 mM EDTA (pH 7.5), i.e., fivefold the standard concentrations, and voltage at 1.0 to develop a protocol which, for the first time, is able to effect an average efficiency of 1.84 × 106 transformants/μg DNA for three commonly used yeast strains committed to two-hybrid screening experiments.
關鍵字
語言 en
ISSN 0003-2697
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Chern, Ming-kai
審稿制度
國別 USA
公開徵稿
出版型式 紙本
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