Inductive properties of polypyridyl ruthenium complexes significantly regulate various protein distributions in Escherichia coli
學年 99
學期 1
出版（發表）日期 2011-01-01
作品名稱 Inductive properties of polypyridyl ruthenium complexes significantly regulate various protein distributions in Escherichia coli
作品名稱（其他語言）
著者 Ho, Ming-yi; Chiou, Min-lun; Du, Wan-shan; Chang, Fang-ying; Chen, Yau-hung; Weng, Yu-jong; Cheng, Chien-chung
單位 淡江大學化學學系
出版者 Philadelphia: Elsevier Inc.
著錄名稱、卷期、頁數 Journal of Inorganic Biochemistry 105(6), pp.902-910
摘要 Ruthenium complexes with similar octahedral structures but different intrinsic inductive properties significantly influence the total cellular protein distributions, which may affect different metabolic pathways. A systematic study of the relationship between ruthenium complexes and Escherichia coli was undertaken, using two-dimensional gel electrophoresis analysis and the identification of various proteins by mass data mining. Based on the low similarities (<40%) between the total protein distributions, the inductive properties of the ruthenium complexes are relevant to the formation of the protein-Ru interaction in addition to the Ru-DNA interaction. Two major protein functions in E. coli BL21 that were reduced by compound 1 were oxidoreductases and transporters, corresponding to 29% and 25% of the 24 down-regulated proteins. The main biological processes of the proteins down-regulated by compound 1 were related to carbohydrate reactions, including in transport, tricarboxylic acid (TCA) cycle, glycolysis, and gluconeogenesis. All four ruthenium complexes shared similar up-regulated proteins, including clpB and kpyk1, and down-regulated similar proteins, including ompA and ybbN. This result supports that the presence of Ru-protein interactions is a major factor affecting bacteria growth, and particularly transport and carbohydrate-related reactions.
關鍵字 Ruthenium;2D gel;Polypyridyl;Metalloproteomic;LC/MS;Ru-protein interaction
語言 en
ISSN 0162-0134
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Cheng, Chien-chung
審稿制度
國別 USA
公開徵稿
出版型式 紙本
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