| Biochemical and structural properties of zebrafish Capsulin produced by Escherichia coli | |
|---|---|
| 學年 | 99 |
| 學期 | 1 |
| 出版（發表）日期 | 2011-01-01 |
| 作品名稱 | Biochemical and structural properties of zebrafish Capsulin produced by Escherichia coli |
| 作品名稱（其他語言） | |
| 著者 | Chou, Chi-yuan; Hsu, Chia-hao; Wang, Yun-hsin; Chang, Min-yen; Chen, Li-chao; Chen, Shu-chun; Chen, Yau-hung |
| 單位 | 淡江大學化學學系 |
| 出版者 | Maryland Heights: Academic Press |
| 著錄名稱、卷期、頁數 | Protein Expression and Purification 75(1), pp.21-27 |
| 摘要 | Capsulin is one of the transcription factors involved in regulating cell differentiation but its biochemical properties and structural characteristics are still unclear. In the present study, we cloned capsulin from zebrafish, which produces large numbers of transparent embryos and has well-characterized developmental stages. By alignment, the deduced amino acid sequence of zebrafish Capsulin, which contains a putative bHLH motif, shares very high homology to that of other species with an 72–82% identity. Zebrafish Capsulin was also targeted to the nucleus of mammalian cells when overexpressed by transient transfection. In order to characterize the structural and biochemical properties of zebrafish Capsulin, a recombinant zebrafish Capsulin protein was expressed and purified in Escherichia coli. By circular dichroism spectroscopy, Capsulin was shown to be 55% α-helical. The size distribution assay by analytical ultracentrifugation indicated that it existed as a monomer–dimer mixture. The results suggested that the recombinant Capsulin has a well-organized and functional structure. Finally, endogenous Capsulin was distributed mainly in the epicardial cells of zebrafish by immunohistochemistry analysis using antibodies raised against zebrafish Capsulin. The present study not only helps us to comparatively analyze capsulin genes across species, but it also provides valuable structural information for further studies of Capsulin biological function in the future. |
| 關鍵字 | Analytical ultracentrifugation;Anti-Capsulin antibody;bHLH structure;Circular dichroism;Recombinant Capsulin;Nuclear localization |
| 語言 | en |
| ISSN | 1046-5928; 1096-0279 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Chou, Chi-yuan; Chen, Yau-hung |
| 審稿制度 | |
| 國別 | USA |
| 公開徵稿 | |
| 出版型式 | 紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/77237 ) |
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