Embryonic exposure to diclofenac disturbs actin organization and leads to myofibril misalignment
學年 99
學期 2
出版（發表）日期 2011-04-01
作品名稱 Embryonic exposure to diclofenac disturbs actin organization and leads to myofibril misalignment
作品名稱（其他語言）
著者 Chen, Ying-hsin; Chang, Chao-yuan; Wang, Yun-hsin; Wen, Chi-chung; Chen, Yun-chen; Hu, Sheng-chuan; Yu, Dah-shyong; Chen, Yau-hung
單位 淡江大學生命科學研究所; 淡江大學數學學系
出版者 Hoboken: John Wiley & Sons, Inc.
著錄名稱、卷期、頁數 Birth Defects Research Part B: Developmental and Reproductive Toxicology 92(2), pp.139-147
摘要 The objective of this study was to investigate the embryotoxicity of diclofenac. Zebrafish (Danio rerio) embryos at 12 hpf were treated with different dosages of diclofenac (0–2,000 ppm) for different time courses (12–72 hr). Results showed no evident differences in survival rates or morphological changes between the mock-treated control (0 ppm) zebrafish embryos and those with 1-ppm diclofenac-exposure (12–24, 12–36 hpf). In contrast, after higher doses (5 and 10 ppm) of exposure, embryos displayed some defective phenotypes, including malformed somite boundary, a twisted body axis, and shorter body length. In addition, diclofenac-treated embryos exhibited significantly reduced frequencies of spontaneous in-chorion contractions in comparison with mock-control littermates (mock-control: 13.20±2.24 vs. 5–10 ppm diclofenac: 6.66±1.35–3.03±1.84). Subtle changes were easily observed by staining with specific monoclonal antibodies F59 and phalloidin to detect morphological changes in muscle fibers and formation of F-actin, respectively. Our data show that diclofenac treatment disturbs actin organization and muscle fiber alignment, thus causing malformed somite phenotypes.
關鍵字 actin;diclofenac;myotoxic;myod;zebrafish
語言 en_US
ISSN 1542-9733; 1542-9741
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Chen, Yau-hung
審稿制度
國別 USA
公開徵稿
出版型式 紙本
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