Inhibition of the P2X7 Receptor Reduces Cystogenesis in PKD | |
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學年 | 100 |
學期 | 1 |
出版(發表)日期 | 2011-09-01 |
作品名稱 | Inhibition of the P2X7 Receptor Reduces Cystogenesis in PKD |
作品名稱(其他語言) | |
著者 | Chang, Ming-yang; Lu, Jenn-kan; Tian, Ya-chung; Chen, Yung-chang; Hung, Cheng-chieh; Huang, Yi-hui; Chen, Yau-hung; Wu, Mai-szu; Yang, Chih-wei; Cheng, Yi-chuan |
單位 | 淡江大學化學學系 |
出版者 | Washington: American Society of Nephrology |
著錄名稱、卷期、頁數 | Journal of the American Society of Nephrology 22(9), pp.1696-1706 |
摘要 | The P2X7 receptor participates in purinergic signaling, which may promote the progression of ADPKD. We examined the effects of a P2X7 receptor antagonist and a P2X7 receptor agonist on cyst development in a zebrafish model of polycystic kidney disease in which we knocked down pkd2 by morpholinos. We used live wt-1b pronephric-specific GFP-expressing zebrafish embryos to directly observe changes in the pronephros. Exposure of pkd2-morphant zebrafish to a P2X7 receptor antagonist (oxidized ATP [OxATP]) significantly reduced the frequency of the cystic phenotype compared with either exposure to a P2X7 receptor agonist (BzATP) or with no treatment (P < 0.01). Histology confirmed improvement of glomerular cysts in OxATP-treated pkd2 morphants. OxATP also reduced p-ERK activity and cell proliferation in pronephric kidneys in pkd2 morphants. Inhibition of P2X7 with an additional specific antagonist (A-438079), and through morpholino-mediated knockdown of p2rx7, confirmed these effects. In conclusion, blockade of the P2X7 receptor reduces cyst formation via ERK-dependent pathways in a zebrafish model of polycystic kidney disease, suggesting that P2X7 antagonists may have therapeutic potential in ADPKD. |
關鍵字 | |
語言 | en_US |
ISSN | 1046-6673 |
期刊性質 | 國外 |
收錄於 | SCI |
產學合作 | |
通訊作者 | Cheng, Yi-chuan |
審稿制度 | |
國別 | USA |
公開徵稿 | |
出版型式 | 紙本 |
相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/77040 ) |