The conversion of group B red blood cells into group O by an α-d-galactosidase from taro (Colocasia esculenta)
學年 80
學期 1
出版（發表）日期 1991-09-18
作品名稱 The conversion of group B red blood cells into group O by an α-d-galactosidase from taro (Colocasia esculenta)
作品名稱（其他語言） 轉變B型紅血球為O型紅血球
著者 Chien, Su-fang; Lin-Chu, Marie
單位 淡江大學化學學系
出版者 Oxford: Pergamon
著錄名稱、卷期、頁數 Carbohydrate research 217, pp.191-200
摘要 An α-d-galactosidase (EC 3.2.1.21), capable of converting group B into group O red cells, was isolated from the stem portion of taro. It was purified about 3000 fold by gel filtration and ion-exchange chromatography. The blood group-converting activity was demonstrated by hemolysis and hemagglutination studies. This activity is comparable to that of α-d-galactosidase isolated from coffee beans. Taro α-d-galactosidase also hydrolyzes (1→4)- and (1→6)-linked α-d-galactopyranosyl groups from d-galactose-containing glycoconjugates. Taro α-d-galactosidase has a low Km value (0.28mM), a low molecular weight (40 000), and a neutral optimal pH (6.0). At a final enzyme concentration of 30 units/mL in the incubation mixture, the conversion of group B into group O activity was completed within two hours, without apparent changes in the shape of the red cells.
關鍵字
語言 en
ISSN 0008-6215 1873-426X
期刊性質 國外
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產學合作
通訊作者
審稿制度
國別 GBR
公開徵稿
出版型式 紙本
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