| 標題：The conversion of group B red blood cells into group O by an α-d-galactosidase from taro (Colocasia esculenta) |
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| 學年 | 80 |
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| 學期 | 1 |
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| 出版（發表）日期 | 1991/09/18 |
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| 作品名稱 | The conversion of group B red blood cells into group O by an α-d-galactosidase from taro (Colocasia esculenta) |
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| 作品名稱（其他語言） | 轉變B型紅血球為O型紅血球 |
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| 著者 | Chien, Su-fang; Lin-Chu, Marie |
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| 單位 | 淡江大學化學學系 |
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| 出版者 | Oxford: Pergamon |
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| 著錄名稱、卷期、頁數 | Carbohydrate research 217, pp.191-200 |
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| 摘要 | An α-d-galactosidase (EC 3.2.1.21), capable of converting group B into group O red cells, was isolated from the stem portion of taro. It was purified about 3000 fold by gel filtration and ion-exchange chromatography. The blood group-converting activity was demonstrated by hemolysis and hemagglutination studies. This activity is comparable to that of α-d-galactosidase isolated from coffee beans. Taro α-d-galactosidase also hydrolyzes (1→4)- and (1→6)-linked α-d-galactopyranosyl groups from d-galactose-containing glycoconjugates. Taro α-d-galactosidase has a low Km value (0.28mM), a low molecular weight (40 000), and a neutral optimal pH (6.0). At a final enzyme concentration of 30 units/mL in the incubation mixture, the conversion of group B into group O activity was completed within two hours, without apparent changes in the shape of the red cells. |
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| 關鍵字 | |
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| 語言 | 英文 |
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| ISSN | 0008-6215;1873-426X |
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| 期刊性質 | 國外 |
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| 國別 | 英國 |
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| 公開徵稿 | |
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| 出版型式 | 紙本 |
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