Subunit fusion of two yeast D-amino acid oxidases enhances their thermostability and resistance to H2O2
學年 97
學期 1
出版（發表）日期 2008-08-01
作品名稱 Subunit fusion of two yeast D-amino acid oxidases enhances their thermostability and resistance to H2O2
作品名稱（其他語言）
著者 Wang, Shih-juei; Yu, Chi-yang; Lee, Cheng-kang; Chern, Ming-kai; Kuan, I-ching
單位 淡江大學生命科學研究所
出版者 Dordrecht: Springer Netherlands
著錄名稱、卷期、頁數 Biotechnology Letters 30(8), pp.1415-1422
摘要 D-Amino acid oxidases from Rhodosporidium toruloides and Trigonopsis variabilis (RtDAO and TvDAO) are both yeast homodimeric flavoenzymes. Two of their cDNA genes were connected by a hexanucleotide linker and heterologously expressed in E. coli to produce the corresponding double DAOs (dRtDAO and dTvDAO) with two subunits fused into a single polypeptide. The specific activities of double DAOs remained similar to those of native dimeric DAOs, although the catalytic efficiencies (kcat/KM) were decreased due to higher KM values. The Tm value for dRtDAO was shifted 5°C higher while that for dTvDAO was increased only by 2°C, in comparison with the corresponding native counterparts. In the presence of 10 mM H2O2, dRtDAO and dTvDAO exhibited half-lives of about 60 and 40 min, respectively, which were 2- and 1.5-fold, respectively, longer than their native DAOs. These yeast DAOs can therefore be thermally and oxidatively stabilized by linking their subunits together.
關鍵字 D-Amino acid oxidase; Gene fusion; Rhodosporidium toruloides; Stability; Trigonopsis variabilis
語言 en
ISSN 0141-5492; 1573-6776
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Kuan, I-ching
審稿制度
國別 NLD
公開徵稿
出版型式 紙本 電子版
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