Purification and biochemical characterization of a nattokinase by conversion of shrimp shell with Bacillus subtilis TKU007
學年 99
學期 2
出版（發表）日期 2011-02-01
作品名稱 Purification and biochemical characterization of a nattokinase by conversion of shrimp shell with Bacillus subtilis TKU007
作品名稱（其他語言）
著者 Wang, San-Lang; Wu, Ying-Ying; Liang, Tzu-Wen
單位 淡江大學化學學系
出版者 Amsterdam: Elsevier BV
著錄名稱、卷期、頁數 New Biotechnology 28(2), pp.196-202
摘要 BSN1, a nattokinase, was purified from the culture supernatant of Bacillus subtilis TKU007 with shrimp shell wastes as the sole carbon/nitrogen source. The BSN1 was purified to homogeneity by three-step procedure with a 515-fold increase in specific activity and 12% recovery. The molecular masses of BSN1 determined by SDS-PAGE and gel filtrations were approximately 30 kDa and 28 kDa, respectively. The results of peptide mass mapping showed that four tryptic peptides of BSN1 were identical to the nattokinase from B. subtilis (GenBank accession number gi14422313) with 37% sequence coverage. The N-terminal amino acid sequence of the first 12 amino acids of BSN1 was AQSVPYGISQIK. The optimum pH, optimum temperature, pH stability, and thermal stability of BSN1 were 8, 40°C, pH 4–11, and less than 50°C, respectively. BSN1 was inhibited completely by PMSF, indicating that the BSN1 was a serine protease. Using this method, B. subtilis TKU007 produces a nattokinase/fibrinolytic enzyme and this enzyme may be considered as a new source for thrombolytic agents.
關鍵字
語言 en
ISSN 1871-6784
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Wang, San-Lang
審稿制度
國別 NLD
公開徵稿
出版型式 紙本
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