Presence of endo-β-N-acetylglucosaminidase and protease activities in the commerical neuraminidase preparations isolated from clostridium perfringens
學年 63
學期 2
出版(發表)日期 1975-07-01
作品名稱 Presence of endo-β-N-acetylglucosaminidase and protease activities in the commerical neuraminidase preparations isolated from clostridium perfringens
作品名稱(其他語言)
著者 Chien, Su-fang; Yevich, Steven J. ; Li, Su-chen; Li,Yu-Teh
單位 淡江大學化學學系
出版者 Philadelphia, PA: Elsevier Inc.
著錄名稱、卷期、頁數 Biochemical and biophysical research communications 65(2), pp.683-691
摘要 Commercial neuraminidase preparations isolated from Clostridium perfringens were found to be contaminated with endoglycosidase and protease activities. The preparations release oligosaccharide fragment(s) with G1cNAc at the reducing end, from the intact ovalbumin. The enzymes also converted Asn-(GlcNAc)2(Man)5 isolated from ovalbumin into Asn-GlcNAc and an oligosaccharide with G1cNAc at the reducing end. Similar results were obtained when Taka-amylase A and its glycopeptide were used as substrates. These results identify the endoglycosidase activity as endo-β-N-acetylglucosaminidase. Furthermore, the commercial neuraminidase preparations contain a protease activity which hydrolyzes Azocoll, hemoglobin, casein, ovalbumin, human serum albumin, and α1-acid glycoprotein.
關鍵字
語言 en
ISSN 0006-291X
期刊性質 國外
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產學合作
通訊作者
審稿制度
國別 USA
公開徵稿
出版型式 ,紙本
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