教師資料查詢 | 類別: 期刊論文 | 教師: 簡素芳CHIEN, SU-FENG (瀏覽個人網頁)

標題:Presence of endo-β-N-acetylglucosaminidase and protease activities in the commerical neuraminidase preparations isolated from clostridium perfringens
學年63
學期2
出版(發表)日期1975/07/01
作品名稱Presence of endo-β-N-acetylglucosaminidase and protease activities in the commerical neuraminidase preparations isolated from clostridium perfringens
作品名稱(其他語言)
著者Chien, Su-fang; Yevich, Steven J. ; Li, Su-chen; Li,Yu-Teh
單位淡江大學化學學系
出版者Philadelphia, PA: Elsevier Inc.
著錄名稱、卷期、頁數Biochemical and biophysical research communications 65(2), pp.683-691
摘要Commercial neuraminidase preparations isolated from Clostridium perfringens were found to be contaminated with endoglycosidase and protease activities. The preparations release oligosaccharide fragment(s) with G1cNAc at the reducing end, from the intact ovalbumin. The enzymes also converted Asn-(GlcNAc)2(Man)5 isolated from ovalbumin into Asn-GlcNAc and an oligosaccharide with G1cNAc at the reducing end. Similar results were obtained when Taka-amylase A and its glycopeptide were used as substrates. These results identify the endoglycosidase activity as endo-β-N-acetylglucosaminidase. Furthermore, the commercial neuraminidase preparations contain a protease activity which hydrolyzes Azocoll, hemoglobin, casein, ovalbumin, human serum albumin, and α1-acid glycoprotein.
關鍵字
語言英文
ISSN0006-291X
期刊性質國外
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產學合作
通訊作者
審稿制度
國別美國
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