Trans-Dioxorhenium(V)-Mediated Electrocatalytic Oxidation of DNA at Indium Tin-Oxide Electrodes: Voltammetric Detection of DNA Cleavage in Solution
學年 83
學期 1
出版（發表）日期 1994-12-01
作品名稱 Trans-Dioxorhenium(V)-Mediated Electrocatalytic Oxidation of DNA at Indium Tin-Oxide Electrodes: Voltammetric Detection of DNA Cleavage in Solution
作品名稱（其他語言）
著者 Johnston, Dean H.; Cheng, Chien-chung; Campbell, Katherine J.; Thorp, H. Holden
單位 淡江大學化學學系
出版者 American Chemical Society
著錄名稱、卷期、頁數 Inorganic chemistry 33(26), pp.6388–6390
摘要 The oxidative electrochemistry of truns-[Re(0)2(4-OMe-py)4]+ in the presence of DNA has been studied. The complex exhibits a reversible oxidation at E1/2(VW) = 1.00 V (vs Ag/AgCl) in buffer or in the presence of poly(dA)poly(dT). However, in the presence of calf thymus DNA or poly(dG)poly(dC), a dramatic catalytic enhancement is observed. An identical result is obtained with Fe(S-C1-~hen)3~+ (E1/2(IIIAI) = 1.02 V), but no electrocatalytic enhancement is observed with truns-[Re(0)2(py)2(dmap)2]+ (E112 = 0.90 V). Electrophoresis of plasmids electrolyzed at 1.2 V in the presence of trans-[Re(0)2(4-OMe-py)4]+ show relaxation from form I to form 11, and analogous reactions with 5'-end 32P-labeled synthetic oligonucleotides show piperidine-labile cleavage specifically at guanine. The combined results point to an electrocatalytic mechanism where the oxidized metal complex oxidizes guanine in DNA by one electron via an efficient, outer-sphere mechanism. Moreover, the experiments demonstrate a potential for the one-electron oxidation of guanine in double-helical DNA at neutral pH of between 0.90 and 1.00 V. This result should provide insight into the mechanisms of DNA oxidation by chemical agents and by ionizing radiation.
關鍵字
語言 en
ISSN 0020-1669 1520-510X
期刊性質 國外
收錄於
產學合作
通訊作者
審稿制度
國別 USA
公開徵稿
出版型式 紙本
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