Electrophoretic behaviors of human hepatoma HepG2 cells
學年 97
學期 2
出版（發表）日期 2009-05-01
作品名稱 Electrophoretic behaviors of human hepatoma HepG2 cells
作品名稱（其他語言）
著者 Hsu, Jyh-Ping; Ku, Ming-Hong; Yang, Li-Chia; Lu, Jui-Nan; Young, Tai-Horng H.; Tseng, Shio-Jenn
單位 淡江大學數學學系
出版者 Weinheim: Wiley - V C H Verlag GmbH & Co. KGaA
著錄名稱、卷期、頁數 Electrophoresis 30(9), pp.1531-1537
摘要 The electrophoretic mobility of HepG2 cells was measured and a charge-regulated model was proposed to simulate the results obtained. Here, a cell was simulated by a rigid core and an ion-penetrable membrane layer containing both acidic and basic functional groups. The influences of the key parameters, including the pH, the ionic strength, the thickness of the membrane layer of a cell, the density and the dissociation constant of the dissociable functional groups in the membrane layer, and the binding constant of divalent cations on the electrophoretic mobility of a cell were investigated. In particular, the role of the buffer used in the experiment was discussed; this effect was neglected in almost all the relevant theoretical analyses in the literature. We showed that the binding ability of divalent cations to the dissociated functional groups in the membrane layer of a cell ranks as Ca2+＞Mg2+＞hexamethonium.
關鍵字 Buffer effect; Electrophoretic mobility; HepG2 cells; Ion-binding; Surface dissociation reaction
語言 en
ISSN 1522-2683
期刊性質 國外
收錄於 SCI EI
產學合作
通訊作者
審稿制度 是
國別 DEU
公開徵稿
出版型式 電子版
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