Development of a label-free impedance biosensor for detection of antibody–antigen interactions based on a novel conductive linker
學年 99
學期 2
出版（發表）日期 2011-02-15
作品名稱 Development of a label-free impedance biosensor for detection of antibody–antigen interactions based on a novel conductive linker
作品名稱（其他語言）
著者 Chen, Ching-sung; Chang, Ku-ning; Chen, Ying-hua; Lee, Chih-kung; Lee, Bryan Yong-jay; Lee, Adam Shih-yuan
單位 淡江大學化學學系
出版者 Amsterdam: Elsevier BV
著錄名稱、卷期、頁數 Biosensors and Bioelectronics 26(6), pp.3072-3076
摘要 We developed a label-free impedance biosensor based on an innovative conductive linker for detecting antibody–antigen interactions. As the often used conventional long chain thiol is a poor conductor, it is not a suitable material for use in a faradaic biosensor. In this study, we adopted a thiophene-based conductive bio-linker to form a self-assembled monolayer and to immobilize the bio-molecules. We used cyclic voltammetry and impedance spectroscopy to verify the enhanced conductivity properties. Results showed that the electron transfer resistance of this new conductive linker was 3 orders of a magnitude lower than for a case using a conventional long chain thiol linker. With the decreased impedance (i.e. increased faradaic current), we can obtain a higher signal/noise ratio such that the detection limit is improved. Using fluorescence microscopy, we verified that our new conductive linker has a protein immobilization capability similar to a conventional long chain thiol linker. Also, using S100 proteins, we verified the protein interaction detection capability of our system. Our obtained results showed a linear dynamic range from 10 ng/ml to 10 μg/ml and a detection limit of 10 ng/ml. With our new conductive linker, an electrochemical impedance biosensor shows great potential to be used for point-of-care applications.
關鍵字 Impedance;Label-free;Conductive linker;S100; Point-of-care
語言 en
ISSN 0956-5663
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Lee, Chih-kung
審稿制度
國別 NLD
公開徵稿
出版型式 紙本
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