| Single-step protein purification by back flush in ion exchange chromatography | |
|---|---|
| 學年 | 98 |
| 學期 | 1 |
| 出版（發表）日期 | 2009-09-01 |
| 作品名稱 | Single-step protein purification by back flush in ion exchange chromatography |
| 作品名稱（其他語言） | |
| 著者 | Chern, Ming-kai; Shiah, Wei-jyh; Chen, Jyun-jie; Tsai, Tzung-you; Lin, Hsin-yin; Liu, Chien-wei |
| 單位 | 淡江大學生命科學研究所 |
| 出版者 | Philadelphia: Elsevier Inc. |
| 著錄名稱、卷期、頁數 | Analytical Biochemistry 392(2), pp.174-176 |
| 摘要 | Ion exchange chromatography, one of the major procedures for protein purification, seldom provides single-step purification due to a lack of specific affinity. In this work, a novel and simple method called “back flush” (i.e., reversing the flow direction of elution relative to that of sample loading) was developed to achieve single-step purification on an ion exchanger. Tips for the conditions and operation by back flush are presented. Our study demonstrates, for the first time, the feasibility and dramatic improvement for protein purification by the back-flush method. |
| 關鍵字 | |
| 語言 | en |
| ISSN | 0003-2697 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | |
| 國別 | USA |
| 公開徵稿 | |
| 出版型式 | 紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/50358 ) |
| SDGS | 產業創新與基礎設施 |
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