Reversible immobilization of lysozyme via coupling to reversibly soluble polymer
學年 92
學期 1
出版（發表）日期 2003-10-08
作品名稱 Reversible immobilization of lysozyme via coupling to reversibly soluble polymer
作品名稱（其他語言）
著者 Sz-Hau Chen; Yue-Horng Yen; Chuan-Lu Wang; San-Lang Wanga
單位 淡江大學生命科學研究所
出版者 Elsevier
著錄名稱、卷期、頁數 Enzyme and microbial technology 33(5), p.643-649
摘要 Hen egg-white lysozyme (HEWL) can be covalently immobilized on a polymeric support (hydroxypropyl methylcellulose acetate succinate, AS-L), which is soluble above pH 5.5 but insoluble below pH 4.5. Commercial preparation of HEWL was used for the immobilization study. The immobilized enzyme (HEWL-AS) has much higher specific activity than those of free HEWL. The effects of pH and temperature on the activity and thermal stability of HEWL-AS were studied and compared with those of free HEWL. HEWL-AS retained nearly half of its initial chitinase activity even after heating at 70 °C for 50 min. The half-life at 4 °C was 4 days for free HEWL and 25 days for HEWL-AS. Following repeated pH cycles between 7 and 4.5, HEWL-AS retained nearly 50% of its original activity after 10 batches of chitinolytic reactions.
關鍵字 Lysozyme;Chitinase;Reversibly soluble polymers;Immobilized enzyme
語言 en
ISSN 0141-0229
期刊性質 國外
收錄於
產學合作
通訊作者
審稿制度 否
國別 USA
公開徵稿
出版型式 ,電子版
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