DNA sequence-specific recognition of peptides incorporating the HPRK and polyamide motifs | |
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學年 | 92 |
學期 | 1 |
出版(發表)日期 | 2004-01-02 |
作品名稱 | DNA sequence-specific recognition of peptides incorporating the HPRK and polyamide motifs |
作品名稱(其他語言) | |
著者 | Chang, Jung-cheng; Yang, Chia-hung; Chou, Ping-yen; Yang, Wan-hsu; Chou, I.-chun; Lu, Ching-tai; Jeng, Kee-ching G; 鄭建中; Cheng, Chien chung; Sheh, Leung |
單位 | 淡江大學化學學系 |
出版者 | Elsevier |
著錄名稱、卷期、頁數 | Bioorganic and medicinal chemistry 12(1), pp.53-61 |
摘要 | Three peptide amides, HPRK(Py)4HPRK-NH2 (PyH-12), HPRK(Py)3HPRK-NH2 (PyH-11) and HPRK(Py)2HPRK-NH2 (PyH-10), incorporating two HPRK motifs and various 4-amino-1-methylpyrrole-2-carboxylic acid residues (Py) were synthesized by solid-phase peptide methodology. The binding of these three peptides to a 5′-32P-labeled 158-mer DNA duplex (Watson fragment) and to a 5′-32P-labeled 135-mer DNA duplex (complementary Crick fragment) was investigated by quantitative DNase I footprinting. On the 158-mer Watson strand, the most distinctive DNase I blockages seen with all three peptides occur around positions 105–112 and 76–79, corresponding to the sequences 5′-GAGAAAAT-3′ and 5′-CGGT-3′, respectively. However, on the complementary Crick strand, only PyH-12 strongly discriminates the 5′-TTT-3′ site around positions 108–110 whereas both PyH-11 and PyH-10 have moderate binding around positions 102–112 comprising the sequence 5′-ATTTTCTCCTT-3′. Possible bidentate and single interactions of the side-chain functions and α-amino protons of the peptides with DNA bases are discussed. Three peptide amides, HPRK(Py)4HPRK-NH2 (PyH-12), HPRK(Py)3HPRK-NH2 (PyH-11) and HPRK(Py)2HPRK-NH2 (PyH-10), were synthesized. On a 5′-32P-labeled 158-mer Watson strand, the most distinctive DNase I footprinting blockages seen with all three peptides occur around sequences 5′-GAGAAAAT-3′ and 5′-CGGT-3′. On the complementary 5′-32P-labeled Crick strand, only PyH-12 strongly discriminates the 5′-TTT-3′ site. Possible interactions of the amino acid side chains of these peptides with DNA bases are discussed. |
關鍵字 | HPRK motif;Polyamide conjugates;Quantitative DNase I footprinting |
語言 | en |
ISSN | 0968-0896 |
期刊性質 | 國內 |
收錄於 | |
產學合作 | |
通訊作者 | |
審稿制度 | 否 |
國別 | TWN |
公開徵稿 | |
出版型式 | ,電子版 |
相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/24940 ) |